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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 4, 2017, pp. 827-835
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Bioline Code: pr17105
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 4, 2017, pp. 827-835
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Lycium barbarum polysaccharide attenuates cisplatin-induced apoptosis in ovary granulosa cells via alleviation of endoplasmic reticulum stress
Huang, Li-Qiong; Zhang, Yuan-Zhen; Zheng, Bo & He, Yi
Abstract
Purpose: To investigate the effect of Lycium barbarum polysaccharide (LBP) on apoptosis of ovary
granulose cells (GCs), as well as its underlying mechanism.
Methods: GCs were isolated from Sprague Dawley (SD) rats and divided into three groups: control
group, model group (DDP) and LBP group. Cell morphology was observed by H & E staining under
optical microscope. Expression of follicle stimulating hormone receptor (FSHR) was assessed by
immunohistochemistry (IHC), while cell viability was assayed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT). Apoptosis was determined by flow cytometry. Expressions of
glucose-regulated protein 78(GRP78), C/EBP homologous protein (CHOP), caspase-3, Bax protein and
B cell lymphoma-2(Bcl-2) were assayed by Western blot and qRT-PCR.
Results: Apoptosis index (37.6 ± 2.44 %) was significantly higher (p > 0.05) in DDP group than in the
control group (14.3 ± 1.09 %), while mRNA levels and expressions of caspase-3, Bcl-2 and Bax
increased significantly (p > 0.05). Expressions of GRP78 and CHOP in the DDP group were also higher
than in the control group (p > 0.05). However, these effects were effectively blocked by co-incubation
with LBP. Moreover, the DDP-induced increase in apoptosis index was dose-dependently and
significantly lowered by LBP (p > 0.05).
Conclusion: LBP exerts protective effect on cisplatin-induced apoptosis in ovary granulosa cells by
alleviating endoplasmic reticulum stress and regulating levels of apoptosis-related proteins. Thus, LBP
has the potential for alleviating adverse effects induced by cisplatin in the treatment of ovary granulosa
lesions.
Keywords
Cis-platin, Ovary granulosa lesions, Lycium barbarum polysaccharide, Endoplasmic reticulum stress, Apoptosis
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