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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 5, 2017, pp. 975-980
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Bioline Code: pr17125
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 5, 2017, pp. 975-980
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Pegylated niosomal nanoparticles loaded with vincristine: Characterization and in vitro evaluation
Mehrabi, Mohammad Reza; Norouzian, Dariush; Shokrgozar, Mohammad Ali; Toliyat, Tayebeh; Chiani, Mohsen; Farhangi, Ali; Alambin, Fariba & Akbarzadeh, Azim
Abstract
Purpose: To investigate the effect of pegylated niosomal vincristine (VCR) on enhanced performance,
drug resistance and prolonged blood circulation time.
Methods: Pegylated niosomal VCR was synthesized by reverse phase evaporation. The mean
diameter, size distribution, and zeta potential of pegylated niosomal VCR were evaluated using a
Zetasizer. The half-maximal concentration (IC50) values of pegylated niosomal VCR and standard VCR
were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The
impact of pegylated niosomal VCR on apoptosis and cell cycle of BCL1 lymphoma cancer cells were
investigated.
Results: The mean diameter, size distribution and zeta potential of pegylated niosomal VCR were 220
nm, 0.4, and –18.8 mV, respectively. Cell proliferation was evaluated using the MTT assay. The IC50
values of pegylated niosomal VCR and standard VCR were 1.6 and 3.5 μg/mL, respectively, after a 24-h incubation. The cytotoxicity of pegylated niosomal VCR was twice that of standard VCR. Furthermore,
flow cytometric analysis of the cell cycle showed that pegylated niosomal VCR induced greater mitotic
arrest than did standard VCR.
Conclusions: The findings demonstrate the effective antitumor activity of pegylated niosomal VCR
compared with standard VCR.
Keywords
Niosome; Anti-tumour; Polyethylene glycol; Vincristine; Encapsulation; Lymphoma
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