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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 5, 2017, pp. 1097-1104
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Bioline Code: pr17141
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 5, 2017, pp. 1097-1104
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Cardioprotective effect of Shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload
Wang, Zhi-Hua; Pan, Jian-Hao; Ma, Xian-Peng; Xu, Xiao-Yun; Yu, Wen-Hui; Fu, Wen-Juan; Ke, Jian; Bi, Chang-Qiong; Wei, Wei; Zhao, Qu; Wang, Feng; Tang, Dan; Ye, Kaihe; Yi, Zhixian & Nie, Hong
Abstract
Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose
Injection (SGI) in rat acute myocardial infarction (AMI).
Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’
consecutive intravenous administration of SGI, serum samples were used to conduct biochemical
analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology
and qPCR studies. Intracellular Ca2+ {(Ca2+)i} overload in H9c2 cells was measured by laser scanning
confocal microscope (LSCM).
Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic
damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram
(ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly
decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression,
but increased Ca2+-Mg2+-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca2+)i overload to protect cells.
Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial
infarct size, restoring ST segment and reversing (Ca2+)i overload. It suggests that SGI may be a new
clinical candidate to treat myocardial infarction.
Keywords
Shenxiong glucose injection; Tanshinol; Ligustrazine; Myocardial infarction; Intracellular Ca2+ overload; Calmodulin; Calmodulin-dependent protein kinase II
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