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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 5, 2017, pp. 1173-1179
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Bioline Code: pr17150
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 5, 2017, pp. 1173-1179
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Guizhi-jia-houpu-xingzi decoction attenuates ovalbumininduced allergic asthma via regulation of Toll-like receptor signal pathway
Zheng, Chuan; Gao, Yongxiang; Zhao, Jing; Liu, Xinglong & Fu, Wen
Abstract
Purpose: To study the effect of Guizhi-jia-houpu-xingzi (GHX) on ovalbumin-induced allergic asthma in
rats.
Methods: An animal model of allergic asthma (AA) in rats was established by intraperitoneal injection
(ip) of ovalbumin (OVA). Thereafter, GHX (375 mg/kg) was administered orally for 7 days. Pulmonary
function, inflammatory cells, immunoglobulin E (Ig) E, interleukin-4 (IL)-4 and interferon-γ (IFN)-γ in
serum and bronchoalveolar lavage fluids (BALF) were determined. Furthermore, mRNA expressions of
Toll-like receptors (TLRs) signal pathway was determined using real time polymerase chain reaction
PCR (q-RT-PCR).
Results: GHX (375 mg/kg) significantly decreased respiratory rate (p < 0.01) and Penh value (p < 0.05)
when compared with AA rats. The inflammatory cells (p < 0.01) and levels of IL-4 (p < 0.01) and IgE (p
< 0.01) were significantly decreased by GHX treatment when compared with AA rats; whereas IFN-γ (p
< 0.05) was significantly increased. Furthermore, GHX significantly decreased the mRNA expressions of
GATA binding protein (GATA)-3 (p < 0.01), TRL-2 (p < 0.01), TRL-4 (p < 0.01), myeloid differentiation
factor 88 (MyD88) (p < 0.01), TNF receptor associated factor 6 (TRAF6) (p < 0.01) and β-arrestin (p <
0.01) in lung tissues, relative to AA rats. However, GHX treatment led to significant up-regulation of
mRNA expression of T-bet (p < 0.01).
Conclusion: These results demonstrate that GHX possesses a potential for treating allergic asthma via
regulation of Toll-like receptor (TLR) signal pathway. They also provide a scientific basis for the
probable use of GHX in clinical treatment of allergic diseases in future.
Keywords
Guizhi-jia-houpu-xingzi decoction; Ovalbumin; Allergic asthma; Toll-like receptor
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