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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 7, 2020, pp. 501-508
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Bioline Code: rm20054
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 7, 2020, pp. 501-508
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Detection of human herpes viruses 1-5 in miscarriage: A case-control study
Charostad, Javad; Mokhtari-Azad, Talat; Yavarian, Jila; Ghavami, Nastaran; Khorrami, Seyed Mahmood Seyed; Behboudi, Emad; Jalilvand, Somayeh; Malekshahi, Somayeh Shatizadeh & Shafiei-Jandaghi, Nazanin Zahra
Abstract
Background: Miscarriage is the spontaneous pregnancy loss before 24 wk of
gestation. The incidence rate of miscarriage over the past few decades has shown
steady or even growing trends. Viral intrauterine infections are one of the probable
etiological causes of miscarriage. Previous evidence have shown that human herpes
viruses (HHVs) could be considered as the potential reasons for intrauterine infections
and adverse pregnancy outcomes.
Objective: This case-control study aimed to detect HHV1-5 DNAs in placental tissues
and assess their association with miscarriage during the first 24 wk of pregnancy in
spontaneous and therapeutic abortions.
Materials and Methods: Placental tissues from 83 women with spontaneous abortions
during the first and the second trimesters of pregnancy and 81 women with therapeutic
abortion during the same gestational age were collected. The DNA extraction was
performed by the phenol/chloroform method. A part of the DNA polymerase gene of
HHVs was amplified with multiplex nested-polymerase chain reaction. The polymerase
chain reaction products were subjected to sequencing.
Results: The results showed the presence of human cytomegalovirus genome in the
placenta of both spontaneous (8.4%) and therapeutic (4.9%) abortions. No statistically
significant differences were found between these two groups. The other investigated
viruses were not detected here.
Conclusion: In conclusion, like some other studies, no correlation was detected
between the HHVs placental infections and the increased risk of spontaneous
abortions. In order to find the actual role of HHVs infections in miscarriage, further
investigations should be performed on a larger sample size in different areas.
Keywords
Spontaneous abortion; Therapeutic abortion; Infections; Human herpes viruses.
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