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Impact of coadministration of apigenin and bone marrow stromal cells on damaged ovaries due to chemotherapy in rat: An experimental study
Talebi, Athar; Roodbari, Nasim Hayati; Sameni, Hamid Reza & Zarbakhsh, Sam
Abstract
Background: Apigenin is a plant-derived flavonoid with antioxidative and antiapoptotic
effects. Bone marrow stromal cells (BMSCs) are a type of mesenchymal stem cells
(MSCs) that may recover damaged ovaries. It seems that apigenin may promote the
differentiation of MSCs.
Objective: The aim of this study was to investigate the effect of coadministration of
apigenin and BMSCs on the function, structure, and apoptosis of the damaged ovaries
after creating a chemotherapy model with cyclophosphamide in rat.
Materials and Methods: For chemotherapy induction and ovary destruction,
cyclophosphamide was injected intraperitoneally to 40 female Wistar rats (weighing
180–200 gr, 10 wk old) for 14 days. Then, the rats were randomly divided into four
groups (n = 10/each): control, apigenin, BMSCs and coadministration of apigenin
and BMSCs. Injection of apigenin was performed intraperitoneally and BMSC
transplantation was performed locally in the ovaries. The level of anti-mullerian
hormone serum by ELISA kit, the number of oocytes by superovulation, the number
of ovarian follicles in different stages by H&E staining, and the expression of ovarian
Bcl-2 and Bax proteins by western blot were assessed after four wk.
Results: The results of serum anti-mullerian hormone level, number of oocytes and
follicles, and Bcl-2/Bax expression ratio showed that coadministration of apigenin and
BMSCs significantly recovered the ovarian function, structure, and apoptosis compared
to the control, BMSC, and apigenin groups (p < 0.001).
Conclusion: The results suggest that the effect of coadministration of apigenin and
BMSCs is maybe more effective than the effect of their administrations individually on
the recovery of damaged ovaries following the chemotherapy with cyclophosphamide
in rats.
Keywords
Apigenin; Bone marrow stromal cells; Chemotherapy; Ovary; Regeneration.
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