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Sulforaphane response on aluminum-induced oxidative stress, alterations in sperm characterization and testicular histomorphometry in Wistar rats
Ogunlade, Babatunde; Adelakun, Sunday & Iteire, Kingsley
Abstract
Background: The exposure of male individual to environmental toxicant is regarded as
a channel that results in reduced sperm counts and infertility.
Objective: This study investigated the ameliorative response of Sulforaphane (SFN) on
Aluminum trichloride (AlCl3) induced testicular toxicity in adult male Wistar rats.
Materials and Methods: A total of 32 adult male Wistar rats (180-200 gm between 8-10
wk) were divided into four groups (n = 8/each). Group A) received distilled water orally
as placebo; Group B) received 100 mg/kgbw AlCl3 only orally; Group C) received 100
mg/kgbw AlCl3 and 100 mg/kgbw SFN orally; and Group D) received 100 mg/kgbw SFN
only orally. After 28 days of experiment, animals underwent cervical dislocation, blood
serum was obtained for analysis, and testes were harvested for biochemical assays,
histology, hormonal profile, and sperm characterization.
Results: The sperm parameters showed a significant difference within the AlCl3 only
group compared with the control and SFN only groups (p = 0.02). However, AlCl3
and SFN co-treatment showed improvement in the motility, viability, and sperm count
compared with the AlCl3 only group (p = 0.02). Furthermore, there was a significant
decline in the levels of hormones profile and antioxidant status in AlCl3 only group
compared to the control and SFN only (p = 0.02). The testicular histoarchitecture of
the AlCl3 only group showed shrinkage of seminiferous tubules, spermatogenesis
disruption, and empty lumen compared to the control and SFN only groups.
Conclusion: The present study revealed the ameliorative response of SFN on
AlCl3-induced testicular toxicity on serum hormone profiles, antioxidant status, lipid
peroxidation, and histomorphometric analysis through oxidative stress.
Keywords
Sulforaphane; Aluminum trichloride; Oxidative stress; Testis; Histology.
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