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Effect of hydroalcoholic extract of Berberis integerrima and resveratrol on ovarian morphology and biochemical parameters in Letrozole-induced polycystic ovary syndrome rat model: An experimental study
Ashkar, Fatemeh; Eftekhari, Mohammad Hassan; Tanideh, Nader; Koohpeyma, Farhad; Mokhtari, Maral; Irajie, Cambyz & Iraji, Aida
Abstract
Background: Resveratrol and Berberis integerrima (B. integerrima) are known to be natural
antioxidants and regulators of human metabolism. However, the effects of resveratrol and B.
integerrima on the ovarian morphology in polycystic ovary syndrome (PCOS) are not obvious.
Objective: This study aimed to determine the effect of the hydroalcoholic extract of B.
integerrima in combination with resveratrol on some biochemical parameters and ovarian
morphology in the letrozole-induced PCOS rat.
Materials and Methods: Seventy adult female Sprague-Dawley rats aged 10-12 weeks weighing
200 ± 20 gr were randomly divided into seven groups (n = 10/each). Group I): normal; Group II):
vehicle; Group III): letrozole-induced PCOS 1 mg/kg letrozole orally, rats receiving 1 cc normal
saline orally; Group IV): PCOS + receiving 150 mg/kg metformin orally; Group V): PCOS +
receiving 20 mg/kg resveratrol orally; Group VI): PCOS + 3 gr/kg barberry orally; and Group VII):
PCOS + receiving 3 gr/kg barberry and 20 mg/kg resveratrol orally. All animals were followedup
for 63 days. The biochemical parameters and histological assessments of ovaries were
performed.
Results: Resveratrol alone and/or in combination with B. integerrima treatment in rats led to
a significant decrease in low-density lipoprotein, triglyceride, malondialdehyde , and tumor
necrosis factor-alpha concentrations (p = 0.02). The groups IV, V, VI, and VII showed a decrease
in insulin resistance and an increase in the superoxide dismutase, total antioxidant capacity, and
high-density lipoprotein (p = 0.01). No significant difference was observed between the level of
serum glucose in the treatment groups. Number of cystic follicles had a significant decrease in
barberry, resveratrol, and their combination groups (p < 0.001).
Conclusion: Resveratrol, B. integerrima, and their combination as natural products with fewer
side effects might be effective as an alternative medicine in treatment of PCOS.
Keywords
Barberry; Resveratrol; Polycystic ovary syndrome; Ovary; Rat.
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