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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 9, 2020, pp. 713-722
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Bioline Code: rm20073
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 9, 2020, pp. 713-722
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Evaluation of vascular endothelial growth factor A and leukemia inhibitory factor expressions at the time of implantation in diabetic rats following treatment with Metformin and Pioglitazone
Zarei, Ronak; Aboutorabi, Roshanak; Rashidi, Bahman; Eskandari, Nahid & Nikpour, Parvaneh
Abstract
Background: Implantation requires intimate crosstalk between the embryo and uterus
for a successful establishment of pregnancy. Type 2 diabetes mellitus may lead to
implantation failure. The effect of diabetes and its therapeutic drugs on implantation is
still largely unclear.
Objective: To assess the endometrial expression changes of vascular endothelial
growth factor A (VEGFA) and leukemia inhibitory factor (LIF), at the time of implantation
in diabetic rats following treatment with Metformin and Pioglitazone.
Materials and Methods: Twenty-eight 6-8-wk-old Wistar female rats weighing 200-250 gr were divided into four groups (n = 7/each). Type 2 diabetes was induced and
Metformin and Pioglitazone were applied for 4 wk. The expression of VEGFA and
LIF was measured by real-time reverse transcription-polymerase chain reaction and
Western blot.
Results: The relative expression of VEGFA transcript was higher in the diabetic
(p = 0.02) and Metformin-treated (p = 0.04) rats compared to the control group.
Furthermore, the VEGFA transcript level significantly reduced in Pioglitazone-treated
diabetic rats (p = 0.03). LIF expression was elevated in the Metformin- and the
Pioglitazone-treated rats and reduced in the diabetic group in comparison with the
control group. Compared to the diabetic rats, the expression of LIF was significantly
elevated in the Metformin- (p = 0.01) and Pioglitazone-treated (p = 0.03) groups.
Conclusion: The expressions of LIF and VEGFA were altered in diabetic rats during
implantation which may be associated with diabetic-related infertility. Pioglitazone is
able to restore the VEGFA and LIF expressions to their baseline levels more efficiently
than Metformin.
Keywords
Embryo implantation; Leukemia inhibitory factor; Vascular endothelial growth factor A; Metformin; Pioglitazone; Rats.
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© Copyright 2020 - Zarei et al. Alternative site location: http://www.ijrm.ir
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