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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 9, 2020, pp. 755-764
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Bioline Code: rm20077
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 9, 2020, pp. 755-764
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Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study
Mohammadzadeh, Masoomeh; khalili, Mohammad Ali; Ramezani, Vahid; Hamishehkar, Hamed; Marvast, Laleh Dehghan; Mangoli, Esmat; Rajabi, Mahya; Sales, Zhima Akhavan & Talebi, Ali Reza
Abstract
Background: Previous studies have examined the effect of resveratrol as a potent
antioxidant for free radicals in semen. While, the prepared spermatozoa are more
affected by ROS factors due to centrifugation and incubation.
Objective: To evaluate the RSV’s effects on the prepared sperm parameters and
chromatin quality in both normozoospermic and asthenozoospermic cases before and
after freezing.
Materials and Methods: The sample of 10 normozoospermic and asthenozoospermic
men was prepared through the swim-up method. The groups were then divided into
two samples of control and experimental (exposure to 30 μmol/l of RSV) to evaluate
and compare the sperm parameters and chromatin quality before and after freezing.
Results: The motility and viability of spermatozoa were seen to be significantly different
before and after freezing separately in the control and treatment samples of the groups
(p ≤ 0.001 and p = 0.001, respectively). However, the stated difference between the
control and treatment samples of normozoospermic and asthenozoospermic patients
were not significant (p > 0.05). In addition, the sperm morphology and chromatin quality
were not significantly different between the two samples of each group; nonetheless,
chromatin quality of the treated sample was better than that of the control before and
after freezing.
Conclusion: Despite the protective effects of RSV on the semen samples, RSV
cannot affect significantly the prepared sperms parameters and chromatin quality in
normozoospermic and asthenozoospermic patients.
Keywords
Resveratrol; Chromatin; Motility; Spermatozoa; Freeze.
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© Copyright 2020 - Mohammadzadeh et al. Alternative site location: http://www.ijrm.ir
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