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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 11, 2020, pp. 961-968
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Bioline Code: rm20096
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 11, 2020, pp. 961-968
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Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study
Norioun, Hamid; Motovali-bashi, Majid & Javadirad, Seyed Morteza
Abstract
Background: The role of KDM3A and its downstream genes in male fertility has
been approved in animal models. Additionally, the expression shrinkage of KDM3A
is significantly correlated with human azoospermia phenotype. Aberrant expression of
micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of
male infertility.
Objective: The aim of this study was to evaluate the expression level of hsa-miR-27a-3p in azoospermic men to reveal its possible association with infertility.
Materials and Methods: This case-control study was conducted on 30 azoospermic
men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive
azoospermia (OA) according to the pathological examinations. Comprehensive
bioinformatics investigations were performed securely and hsa-miR-27a-3p was
selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RTqPCR)
method was used and statistical analysis was performed to compare the
expression level of hsa-miR-27a-3p in both OA and NOA individuals.
Results: In silico analysis suggested hsa-miR-27a-3p, with its potential binding ability
to target KDM3A transcripts. The expression analysis of candidate hsa-miR-27a-3p
indicated its significant overexpression in NOA men.
Conclusion: The hsa-miR-27a-3p was overexpressed in NOA men compared
to OA-control individuals. As a consequence, the overexpressed micro-RNA
could downregulate directly KDM3A and indirectly TNP1 and PRM1. Therefore,
spermatogenesis could be misled and male infertility could be developed.
Keywords
hsa-miR-27a-3p; Male infertility; KDM3A.
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