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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 12, 2020, pp. 1019-1028
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Bioline Code: rm20110
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 12, 2020, pp. 1019-1028
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The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study
Adib, Maryam; Seifati, Seyed Morteza; Ashkezari, Mahmood Dehghani; Khoradmehr, Arezoo; Rezaee-Ranjbar-Sardari, Roshan; Tahajjodi, Somayyeh Sadat & Aflatoonian, Behrouz
Abstract
Background: To increase the results of infertility treatment, many efforts have been made
to improve the treatment methods. As assisted reproductive technology is mainly using
cell culture methods, one of the approaches to improve this technology is conditioned
medium from different sources. It is desirable to apply in vitro maturation (IVM) and use
oocytes from normal cycles instead of stimulating ovulation.
Objective: To investigate the effect of human cumulus cell condition medium (hCCCM)
on the IVM of immature mouse oocytes and morphology.
Materials and Methods: In this experimental study, 240 germinal vesile oocytes were
collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin
(PMSG) injection and cultured in hCCCM (test group, n = 120) and DMEM + 20% FBS
(control group, n = 120). The IVM rates and changes in perivitelline space (PVS) and shape
were investigated at 8, 16, and 24 hr following the culture. The mature (MII) oocytes were
subjected to in vitro fertilization (IVF) and the fertilization rate was assessed in three days.
Results: A significant difference was observed between the maturation rates in the
hCCCM and control groups (24.16% vs 0%; p = 0.001), as well as morphologic changes
between the two groups (p = 0.04, p = 0.05). The development rate for MII oocytes
attained from IVM in the hCCCM group was 27.58% (2-cell) and 6.89% (4-cell). Data
displayed that hCCCM is an effective medium for oocytes maturation compared to the
control medium.
Conclusion: hCCCM supports oocyte in vitro growth and maturation. Moreover, hCCCM
changes the oocyte shape and size of perivitelline space.
Keywords
Germinal vesicle; Cumulus cell; Conditioned medium; In vitro fertilization; In vitro maturation; Oocyte.
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