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Protective properties of Rydingia persica in reproductive complications induced by diabetes in male rats: An experimental study
Ostovan, Fereshteh; Gol, Ali & Javadi, Abdolreza
Abstract
Background: In Iranian traditional medicine, Rydingia persica (R.P) is commonly used
to treat diabetes mellitus (DM).
Objective: We assessed the protective effects of R.P against testis and epididymis
oxidative stress and the hormonal changes induced by DM.
Materials and Methods: Forty male Wistar rats (12 wk old) weighing 230-270
gr were divided into five groups (n = 8/each): 1. Control (C); 2. diabetic (D); 3.
diabetic + R.P200 (D+R200); 4. diabetic + R.P400 (D+R400); and 5. diabetic +
R.P600 (D+R600). Groups C and D received 2 ml of normal saline orally daily for
two wk and groups D+R200, D+R400, and D+R600 received 200, 400, and 600
mg/kg body weight of R.P powder, respectively, orally daily for two wk. DM was
induced by a single intraperitoneal injection of streptozotocin at 60 mg/kg body
weight. We assessed malondialdehyde, glutathione peroxidase, glutathione reductase,
superoxide dismutase, catalase, hydrogen peroxide, and glutathione in both the testis
and epididymis and also the histological changes of the testis.
Results: Diabetic rats showed a significantly increased and decreased level of
oxidant and antioxidant factors, respectively, and a significantly lower level of serum
testosterone and luteinizing hormone than the control group. In the histological study
of the testis, deteriorations were observed. Treatment with R.P reversed these changes
toward the state of the control group with the highest effectiveness shown by group
D+R600.
Conclusion: The data obtained suggest that R.P powder has antioxidant effects on
testis and epididymis tissues in diabetic rats and that it improves histological testicular
structure in diabetics. It can also correct testosterone and luteinizing hormone changes
induced by DM.
Keywords
Diabetes mellitus; Rydingia persica; Oxidative stress; Reproductive; Testosterone
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