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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 6, No. 4, 2009, pp. 597-604
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Bioline Code: st09066
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 6, No. 4, 2009, pp. 597-604
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Preliminary investigation of chloramphenicol in fish, water and sediment from freshwater aquaculture pond
Lu, X. W.; Dang, Z. & Yang, C.
Abstract
Analytical methods of chloramphenicol in the aquaculture environment have been developed using
high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. The contents of
chloramphenicol were determined using high-performance liquid chromatography for sediment and liquid
chromatography-tandem mass spectrometry for fish and water collected from a freshwater aquaculture pond in China.
Chloramphenicol in the water and sediment were 112.3 ng/L and 0.1957 mg/kg, respectively. The chloramphenicol
residues in 3 kinds of fish, including carp, chub and grass carp were different. Only the muscle and head of grass carp
were under the minimum required performance limit (0.3 μg/kg) and were safe to eat. The chloramphenicol in other
tissues of grass carp, carp and chub exceeded the minimum required performance limit. The highest content of
chloramphenicol was in the branchia of carp and the lowest was in the head of grass carp. The results showed the
chloramphenicol in the aquaculture environment was serious, although the government of China had banned the use of
chloramphenicol in aquaculture a few years ago.
Keywords
Aquaculture environment; Content analysis; Enrichment; Liquid chromatography-mass spectrometry; High-pressure liquid chromatography; Safe assessment
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