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Extraction of extracellular polymeric substances from activated sludge using sodium oxalate
Sajjad, M. & Kim, K. S.
Abstract
In this study, sodium oxalate was employed to
extract extracellular polymeric substances (EPS) from
activated sludge samples. The optimum dose of sodium
oxalate was 1.34 g/g VSS (volatile suspended solids), and
the extraction time was 60 min at pH 7. The total EPS
contents obtained under the optimized conditions were
approximately 191 mg/g VSS, and they predominantly
consisted of proteins, polysaccharides and humic substances.
The extraction efficiencies of the chosen method
for the major biopolymers were 39, 43 and 410 % higher
compared with the ethylenediaminetetraacetic acid
(EDTA), cation exchange resin and control methods. Cell
lysis measured in terms of deoxyribonucleic acid, and
unidentified EPS were approximately 50 % lower in the
sodium oxalate method than in the NaOH and EDTA
methods. The EPS composition and the ratio of proteins/
polysaccharides (1.38–2.21) were extremely dependent on
the operating conditions maintained during the extraction.
The inductively coupled plasma spectroscopic results
demonstrated that the release of divalent metals, such as
Ca2+ and Mg2+, from the sludge matrix was directly correlated
with higher EPS extraction efficiencies in the
sodium oxalate protocol. Moreover, the precipitation of
Ca2+ and Mg2+ as metallic oxalates disrupted the floc
structure and subsequently enhanced the EPS recovery.
Fourier transform infrared studies revealed the presence of
functional groups due to EPS molecules in all extracted
samples.
Keywords
Cation bridging; Fourier transform infrared characterization; Ion exchange; Process optimization; Quantification
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