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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-1472
Vol. 13, No. 7, 2017, pp. 1823-1832
Bioline Code: st16170
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 13, No. 7, 2017, pp. 1823-1832

 en Influence of varying nitrogen levels on lipid accumulation in Chlorella check for this species in other resources sp.
Kiran, B.; Pathak, K.; Kumar, R.; Deshmukh, D. & Rani, N.

Abstract

Microalgal lipids can be enhanced through varying nitrogen (N) content, and limited supply of nitrogen source seems to be valuable approach for increased lipid accumulation in microalgae. In this study, Chlorella sp. IM-02 was observed under fluorescence microscope for increased number of lipid bodies under nitrogen scarcity. Fourier transform infrared spectroscopy was used to determine spectral changes due to varying lipid content under nitrogen-starved (N0, without sodium nitrate), nitrogen-limited (N0.1, N0.25, N0.5 and N1.0 representing 0.1, 0.25, 0.5 and 1.0 g/L of sodium nitrate, respectively) and nitrogen-sufficient (N1.5, i.e., 1.5 g/L sodium nitrate) setting. Chlorophyll content was also monitored under these conditions as growth indicator. Various biochemical components viz. total carbohydrates, total proteins and total lipids were also estimated under varying nitrogen levels spectrophotometrically. On fourth day itself, maximum lipid productivity was observed in case of N0.5, which is having one-third of nitrogen concentration present in original growth media, BG-11. This concludes N0.5 as suitable nitrogen provision for better production of lipids in Chlorella sp. IM-02 without much compromising the biomass production as both growth and lipid quantity are key parameters affecting the lipid productivity of any microalgal strain.

Keywords
Microalgae; Nutrient stress; Wastewater; Fluorescence microscopy

 
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