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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 13, No. 10, 2016, pp. 2135-2142
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Bioline Code: st16214
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 13, No. 10, 2016, pp. 2135-2142
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Removal of anthropogenic lead pollutions by a potent Bacillus species AS2 isolated from geogenic contaminated site
Cephidian, A.; Makhdoumi, A.; Mashreghi, M. & Mahmudy Gharaie, M. H.
Abstract
A total of 422 bacterial isolates were obtained
from the lead (Pb) ore in north-eastern Iran. The Pb tolerances
of these strains were studied using microbroth
serial dilution approach and 35 strains could grow up to
3250 ppm Pb concentration. Of these strains, 10 of them
represented qualitatively high levels of Pb adsorption and
were selected for quantitative studies. Strain AS2 which is
phylogenetically related to genus Bacillus showed the
highest level of Pb remediation. The effects of different
factors, including pH, initial Pb concentration, temperature
and inoculum size, were studied on the remediation process.
Pb remediation capacity was reached at 74.5 mg/g
(99.5 % of initial Pb) at pH 4.5, temperature 30 °C,
inoculum size 1.0 % (v/v) and an initial Pb concentration
of 500 ppm after 24 h. Pb desorption capacity of strain was
66 %. The novel isolate could remove 98 % of Pb from the
contaminated industrial wastes after 24 h. Pb uptaking to
the cell surface was proven using scanning electron
microscopic micrograph and energy-dispersive X-ray
spectroscopy analysis. Most Pb removal efficiency was
observed in the active cell culture as compared to the
inactive cell and extracellular polymeric substances. The
novel strain represents a good candidate for removal of
environmental anthropogenic Pb pollutions.
Keywords
Anthropogenic; Bioremediation; Geogenic; Lead (Pb)
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