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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 9, No. 1, 2012, pp. 1-7
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Bioline Code: tc12001
Full paper language: English
Document type: Research Article
Document available free of charge
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African Journal of Traditional, Complementary and Alternative Medicines, Vol. 9, No. 1, 2012, pp. 1-7
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EFFECTS OF TRADITIONAL CHINESE MEDICINE WEI-WEI-KANG-GRANULE ON THE EXPRESSION OF EGFR AND NF-KB IN CHRONIC ATROPHIC GASTRITIS RATS
Lin, Hai-yan; Zhao, Yan; Yu, Jia-ning; Jiang, Wei-wei & Sun, Xi-ling
Abstract
Wei-Wei-Kang-Granule(WWKG) is a traditional Chinese medicine (TCM) preparation for the treatment of
chronic atrophic gastritis (CAG). We examined the pathologic change and the effects of Wei-Wei-Kang-Granule (WWKG)
on the expression of EGFR (epiderminal growth factor receptors) and NF-kB (nuclear transcription factor KappaB) in rats
with chronic atrophic gastritis (CAG), and evaluated the possible mechanisms. Ninety rats were randomly divided into
control group and four experimental groups. CAG rat models were induced by repeated stimulating experiments in the
experimental groups. After modeled rats were intragastrically injected (i.g.) with WWKG at 6000mg/kg (large dose WWKG
group), WWKG at 3000mg/kg (small dose WWKG group), San-Jiu-Wei-Tai-Granule(SJWTG) at 1600mg/kg(SJWTG
group), and normal saline(0.9%)at 20ml/kg (model group and control group), respectively, once a day for 30 days. After 30
days, all rats were sacrificed and samples were taken from the sinus ventriculi and body of stomach. The gastric specimens
were prepared for microscopic view with hematoxylin and eosin (H-E). The immunohistochemistry method was used to
observe the expression of protein of EGFR and NF-kB in gastric tissue. The data were analyzed in pre- and post-treatment by
computer image automatic analysis system. Immunohistochemistry detection showed that the average optical density of
EGFR and NF-kB in antrum was lower in large and small dose WWKG groups than the model group (P<0.01). CAG in rats
was related with the damage of barrier in gastric mucosa and the misbalance of cell proliferation and apoptosis. One of the
mechanisms is perhaps to reduce the expressing of EGFR and NF-Kb in gastric mucosa.
Keywords
Chronic atrophic gastritis(CAG); EGFR,NF-kB
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