This study identified flavonoid glycosides in species of the genus
Alchemilla
,
A. procerrima
,
A. stricta
,
A.
hirtipedicellata
and
A. sericata
.
A. procerrima is an endemic species for Turkey. After detailed investigation, flavonoid
compounds of the species were identified for the first time. In this study, flavonoid compounds were determined by using two
different chromatography techniques, TLC and HPLC. The following flavonoid compounds were identified from the
Alchemilla species studied. They are as follows: orientin (luteolin-8-
C-glucoside)
Rf: 0,70, vitexin (apigenin-8-
C-glucoside)
Rf: 0,77 as flavone-
C-glycoside, rutin (quercetin-3-
O-rutinoside)
Rf: 0,44, hyperoside (quercetin-3-
O-galactoside)
Rf: 0,65,
isoquercetin (quercetin-3-
O-glucopyranoside)
Rf: 0,72, quercitrin (quercetin-3-
O-rhamnoside)
Rf: 0,84 as flavonol-
O-glycoside.
Three more folavonoids with
Rf values of
Rf1= 0,36,
Rf2=0,54 and
Rf3=0,68 were also identified for the first time
in this study. Rutin (quercetin-3-O-rutinoside) and the flavonoid glycoside, shown as
Rf2 were found in all species. Quercitrin
and isoquercetin were determined in all analysed species but
A. procerrima. Hyperoside was identified in all species except
for
A. stricta. Vitexin was determined only in
A.stricta. Orientin was determined in
A. procerrima and
A. stricta, but could
not be determined in
A. sericata and
A. hirtpedicellata. Unknown flavonoid with
Rf1 and
Rf3 were determined outside of
A.
sericata. Description of these compounds in Turkish
Alchemilla plants for the first time should be viewed as a discovery of
an important chemosystematic feature.
