The aim of this study was to investigate
in vitro antioxidant, anti-inflammatory and cytotoxic activities of the petroleum ether, ethyl acetate, methanol and aqueous extracts obtained from leaves of
Drypetes sepiaria
(Euphorbiaceae). Total phenolic and flavonoid contents of these crude extracts were determined as gallic acid and quercetin equivalents, respectively. In
in vitro antioxidant method, methanol extract exhibited higher free radical scavenging activity compared to standard compound, ascorbic acid with IC
50 of 95.43μg/ml (DPPH) and 67.05μg/ml (ABTS). Methanol extract was able to inhibit inflammation by
in vitro about 85-90% (HRBC stabilization method) and
in vivo about 40-45% (Paw oedema method) anti-inflammatory assays compared to standard produced 50.04% at 6h period. In cytotoxicity assay (MTT assay) methanolic extract exhibited IC
50 of 10μg/ml. In apoptosis (flow cytometric assay), the control group showed normal caspase 3 activity in the SiHa cells which was 0.24%, and increased up to 40% after treatment.
