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PHARMACOLOGICAL EXPERIMENTAL STUDY OF THE ANTI-DEPRESSANT EFFECT OF TOTAL SAIKOSAPONINS
Liu, Yu; Cao, Chunmei & Ding, Haijun
Abstract
Background: Chai Hu has the hepato-protective, choleretic, anti-tussive, analgesic, anti-inflammatory, anti-viral, hypotensive, hypolipidemic,
and anti-tumor pharmacological effects. In this study, the objective of this paper was to study the anti-depressant effect of total saikosaponins.
Materials and Methods: Total saikosaponins were extracted by reflux method, and were identified by thin layer chromatography (TLC). The
anti-depressant effect of total saikosaponins was investigated in vitro by tail suspension test, forced swimming test, and reserpine antagonism test
in mice.
Results: Two times of reflux extraction, temperature of 70℃, and extraction time of 4hrs, for each extraction could improve the yield of
saikosaponins. Each treatment group (100, 200, and 300 mg/kg), could significantly shorten the immobility time of mice in the tail suspension test
in a somewhat dose-dependent manner. The total saikosaponins antagonized the reserpine-induced akinesia, and ptosis in mice.
Conclusion: Total saikosaponins have an anti-depressant effect.
Keywords
total saikosaponins; tail suspension test; forced swimming test
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