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THE ANTI-OXIDANT EFFECTS OF GINGER AND CINNAMON ON SPERMATOGENESIS DYS-FUNCTION OF DIABETES RATS.
Khaki, Arash; Khaki, Amir Afshin; Hajhosseini, Laleh; Golzar, Farhad Sadeghpour & Ainehchi, Nava
Abstract
Background: Diabetes rats have been linked to reproductive dysfunction and plant medicine has been shown to be effective in its treatment. Anti-oxidants
have distinctive effects on spermatogenesis, sperm biology and oxidative stress, and changes in anti-oxidant capacity are considered to be
involved in the pathogenesis of chronic diabetes mellitus. Ginger and cinnamon are strong anti-oxidants and have been shown to reduce oxidative
stress in the long-term treatment of streptozotocin (STZ)-induced diabetes in animal models. The present study examined the influence of combined
ginger and cinnamon on spermatogenesis in STZ-induced diabetes in maleWistar rats.
Materials and Methods: Animals (n = 80) were allocated randomly into eight groups, 10 each: Group 1: Control rats given only 5cc Normal saline
(0.9% NaCl) daily;Group2: rats received ginger (100mg/kg/rat) daily; Group 3: rats received cinnamon (75mg/kg) daily; Group 4: rats received ginger
and cinnamon, (100mg/kg/rat ginger and 75mg/kg cinnamon) daily; Group 5: Diabetic control rats received only normal saline. Group 6: Diabetic
rats received 100mg/kg/day ginger; Group 7: Diabetic rats received 75mg /kg/ day cinnamon; Group 8: Diabetic rats received ginger and cinnamon
(100mg/kg/day and 75mg/kg /day). Diabetes was induced with 55 mg/kg, single intra-peritoneal injection of STZ in all groups. At the end of the
experiment (56th day), blood samples were taken for determination of testosterone, LH,FSH, total anti-oxidant capacity, and levels of
malondialdehyde, SOD, Catalase and GPX. All rats were euthanized, testes were dissected out and spermatozoa were collected from the epididymis
for analysis.
Results: Sperm numbers, percentages of sperm viability and motility, and total serum testosterone increased in ginger and cinnamon and combined
ginger and cinnamon treated diabetic rats compared with control groups. Serum testosterone, LH and FSH were higher compared to control group and
also serum anti-oxidants (TAC, SOD, GPX and catalase) all were increased at the end of treatment. Combined ginger and cinnamon showed more
intense increase in all parameters compare to ginger and cinnamon alone. Most of the results were significant (P<0.05).
Conclusion: We concluded that combined ginger and cinnamon have significant beneficial effects on the sperm viability, motility, and serum total
testosterone, LH,FSH and serum anti-oxidants’ level and could be effective for maintaining healthy sperm parameters and male reproductive function
in diabetics.
Keywords
Ginger; Cinnamon; Streptozotocin; Spermatogenesis; rat
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