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Tsinghua Science and Technology
Tsinghua University, China
ISSN: 1007-0212
Vol. 6, No. 3, 2001, pp. 243-247
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Bioline Code: ts01074
Full paper language: English
Document type: Research Article
Document available free of charge
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Tsinghua Science and Technology, Vol. 6, No. 3, 2001, pp. 243-247
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Conformational Changes in HL60 Cells during Differentiation and Apoptosis Induced by Genistein
WANG Zhao, ZHOU Jiangbing, SUN Suqin, LIU Mingjie , ZHANG Hongjun
Abstract
Genistein can induce not only differentiation but also apoptosis in HL60 human leukemia cells. During the differentiation and apoptosis, the HL60 cells undergo some conformational changes. Fourier transform infrared (FT-IR) was employed to detect those changes. The results showed that the contents of DNA/protein and glycoprotein/protein increased. The α-helix of the membrane protein also increased. In addition, the JG(CZJYOJG) (H) stretching mode of serine, threonine and tyrosine residues of the cell proteins also changed. Those conformational changes suggest some mechanisms for how genistein affects the HL60 cells.
Keywords
genistein; HL60; conformational change; differentiation; apoptosis
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