Samples of
Gekko gecko were collected from 12 areas of Guangxi China, Vietnam and Laos. Four hundred and twenty four base pairs of
G. gecko mitochondrial Cyt
b gene were sequenced and seven haplotypes were found.
Gekko vittatus
and
Teratoscincus keyserlingii
acting as outgroups, the phylogenetic trees of the
G. gecko constructed using NJ and MP methods showed that the average genetic distances were: 0.20%-1.20% between four haplotypes of black
G. gecko from Guangxi China; 0.50% between Vietnamese and Laotian red
G. gecko; 1.70% between Ningming and Vietnamese red
G. gecko; 2.20% between Guangxi and Laotian red
G. gecko; 8.60%-9.50% between black
G. gecko from Guangxi and all red
G. gecko. The genetic difference of black and red
G. gecko reached the
species or subspecies differentiation.