The creatine kinase（CK） cDNA from the mandarin fish
Siniperca chuatsi
was cloned by RT-PCR and
rapid amplification of cDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were
analyzed. Sequence analysis revealed a 1 586 bp cDNA sequence containing 92 bp 5′-untranslated region, 348 bp
3′-untranslated region and 1146 bp open reading frame (ORF), which encoded 381 amino acids. Conserved sequence
blocks of vertebrate CKs and diagnostic boxes for the muscle CK（M-CK） isozyme were identified in
S. chuatsi CK.
Siniperca chuatsi CK showed a higher similarity with vertebrates M-CK isozyme than other CK isozymes (Brain CK,
Mitochondrial CKs) and grouped with M-CK isozyme in CK phylogeny, which strongly supported that
S. chuatsi CK
belongs to M-CK isozyme type. Semi-quantitative RT-PCR analysis demonstrated that the M-CK transcript expression
varied among the different tissues and was detected at a high level in skin, ovary, kidney, stomach, muscle and heart, but
lower in eye, brain and liver.
