At present，transcription analysis of gene expression commonly uses housekeeping genes as control for
normalization．In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S rRNA
in six tissues and five developmental stages of the Mandarin fish
Siniperca chuasti
were assayed with quantitative
real-time PCR (qPCR)．Differences in expression levels were analyzed using geNorm program. The results demonstrate
that β-actin is the most stable gene at developmental stages and GAPDH is the most stable in different tissues. While 18S
rRNA expression during development is differentially regulated, which indicates it is suitable as an internal control for
gene expression normalization at the developmental level. Overall, the data suggest that the two most stable housekeeping
genes are enough to accurately calibrate gene expression in
S. chuatsi. The significance of this study provided convincing
references and methodology for housekeeping gene selection and normalization in gene expression analysis with regular
PCR or qPCR.
