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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 3, 2010, pp. 261-267 		Bioline Code: zr10034
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 3, 2010, pp. 261-267

 en cDNA Cloning, Characterization and mRNA Expression of a Profilin from the Swimming Crab Portunus trituberculatus check for this species in other resources
SHEN, Wang; YE, Mao; SHI, Ge & WANG, Ri-Xin

Abstract

We isolated and characterized the profilin full-length cDNA from hemocytes of swimming crab Portunus trituberculatus check for this species in other resources . The profilin cDNA consists of 742 bp and encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. The deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito Anopheles gambiae check for this species in other resources . The profilin mRNA was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. The higher expression of profilin mRNA is observed in crab challenged by the pathogenic bacterium Vibrio parahaemolyticus check for this species in other resources . These results suggest a potential role for profilin in pathogen host defense mechanisms.

Keywords
Portunus trituberculatus; Immunity; Gene cloning; Profilin; Gene expression

 
© Copyright 2010 Kunming Institute of Zoology, the Chinese Academy of Sciences
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