The complete sequence of
Pseudopodoces humilis
mitochondrial genome was determined by using long PCR and conserved primers walking approaches. The results showed that the entire mitochondrial genome of
Pseudopodoces humilis was 16 809 bp in length with 52.9% A+T content, and the 37 genes had the same gene order with that of Gallus gallus. All protein coding genes of the Pseudopodoces humilis mitochondrial genome started with ATG codon, except for
COIwith GTG. For terminate codon usage, most of genes terminate with codons TAA or TAG,
ND1 and
ND5 were AGA,
COII was AGG, and the COIII and
ND4 genes had an incomplete termination codon (T). The secondary structures of 22 tRNA were predicted, all tRNA can form typical secondary structure, except tRNA
Ser ( AGN ) with an absence of DHU arm, and tRNA
Phe with an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helics in 3 structural domains and 60 helics in 6 domains, respectively. The control region of
Pseudopodoces humilis located between tRNA
Glu and tRNA
Phe with 1 240 bp in length, and has structure domains found in that of the other birds.
