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Effects of some extenders and monoamines on sperm cryopreservation in tree shrews ( Tupaia belangeri )
PING, Shu-Huang; WANG, Cai-Yun; TANG, Wen-Ru; LUO, Ying & YANG, Shi-Hua
Abstract
The tree shrew may be an important experimental animal for disease models in humans. The effects of
some extenders and momamines on sperm cryopreservation will provide helpful data for experimentation of strains and
conservation of genetic resources in tree shrews. Epididymal sperm were surgically harvested from male tree shrews
captured around Kunming, China and sperm motility, acrosome integrity and fertility were assessed during
cryopreservation. In Experiment 1 eight extenders (TTE, TCG, TCF, TTG, BWW, BTS, DM, and SR) supplemented with
0.4 mol/L DMSO were used to dilute the sperm: only TTE, DM and SR showed no differences in motility and acrosome
integrity compared to fresh controls after equilibration. After freezing and thawing, sperm in any extender showed lower
motility than fresh control and sperm in DM showed higher motility than other groups. However, BWW produced the
lowest motility. For acrosome intergrity, TTE and DM showed higher than BWW, BTS and SR after equilibration. The
parameter in DM was higher than other groups (except TTE) after thawing. In Experiment 2 four penetrating
cryoprotectant agents (CPA) [dimethyl-formamide (DF), formamide (F), dimethylacetamide (DA), and acetamide (A)] at
0.2 mol/L, 0.4 mol/L, 0.8 mol/L, and 1.2 mol/L, respectively were added to the DM extender. Motility showed no
difference among CPA groups and non-CPA group (control) after equilibration, but all thawed sperm showed lower
values in motility and acrosome intergrity than pre-freezing groups. However, sperm in 0.8 mol/L DF and 0.4 mol/L DMSO showed higher values in both parameters than that in other CPA groups (P>0.05). In Experiment 3 the
fertilization rate of oocytes inseminated with 0.4mol/L DMSO (50%) were higher than that with 0.8mol/L DF (16%). In
conclusion, non-ion extenders supplemented with egg yolk may be better for sperm cryopreservation in tree shrews and
cryoprotectant effects of monoamines agents should be further studied in this species.
Keywords
Tree shrew; Sperm cryopreservation; Extender; Monoamines
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