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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 4, 2012, pp. 389-394
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Bioline Code: zr12062
Full paper language: English
Document type: Research Article
Document available free of charge
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Zoological Research, Vol. 33, No. 4, 2012, pp. 389-394
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Phylogenetic analysis and expression patterns of tropomyosin in amphioxus
LI, Xin-Yi; LIN, Yu-Shuang & ZHANG, Hong-Wei
Abstract
In amphioxus, we found a mesoderm related gene, tropomyosin, which encodes a protein comprising 284
amino acid residues, sharing high identities with other known Tropomyosin proteins both in vertebrates and invertebrates.
Phylogenetically, amphioxus Tropomyosin fell outside the invertebrate clade and was at the base of the vertebrate protein
family clade, indicating that it may represent an independent branch. From the early neurula to the larva stage,
whole-mount in situ hybridization and histological sections found transcripts of amphioxus tropomyosin gene. Weak
tropomyosin expression was first detected in the wall of the archenteron at about 10 hours-post-fertilization neurula stage,
while intense expression was revealed in the differentiating presumptive notochord and the muscle. Transcripts of
tropomyosin were then expressed in the formed notochord and somites. Gene expression seemed to continue in these
developing organs throughout the neurular stages and remained till 72-hours, during the early larval stages. In situ study
still showed tropomyosin was also expressed in the neural tube, hepatic diverticulum, notochord and the spaces between
myotomes in adult amphioxus. Our results indicated that tropomyosin may play an important role in both embryonic
development and adult life.
Keywords
Amphioxus; Phylogenetic analysis; Embryogenesis; Expression pattern; Tropomyosin
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