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Polymorphisms of inhibin α gene exon 1 in buffalo ( Bubalus bubalis ), gayal ( Bos frontalis ) and yak ( Bos grunniens )
MIAO, Yong-Wang; HA, Fu; GAO, Hua-Shan; YUAN, Feng; LI, Da-Lin & YUAN, Yue-Yun
Abstract
To elucidate the genetic characteristics of the bovine Inhibin α subunit (INHA) gene, the polymorphisms
in exon 1 of INHA and its bilateral sequences were assayed using PCR with direct sequencing in buffalo, gayal and yak. A
comparative analysis was conducted by pooled the results in this study with the published data of INHA on some
mammals including some bovine species together. A synonymous substitution c.73C>A was identified in exon 1 of INHA
for buffalo, which results in identical encoding product in river and swamp buffalo. In gayal, two non-synonymous but
same property substitutions in exon 1 of INHA, viz. c.62 C>T and c.187 G>A, were detected, which lead to p. P21L, p.
V63M changes in INHA, respectively. In yak, nucleotide substitution c.62C>T, c.129A>G were found in exon 1 of INHA,
the former still causes p. P21L substitution and the latter is synonymous. For the sequence of the 5'-flanking region of
INHA examined, no SNPs were found within the species, but a substitution, c. -6T>G, was found. The nucleotide in this
site in gayal, yak and cattle was c. -6G, whereas in buffalo it was c. -6T. Meanwhile, a 6-bp deletion, namely c. 262+31_262+36delTCTGAC, was found in the intron of buffalo INHA gene. For this deletion, wild types (+/+) account
for main part in river buffalo while mutant types (-/-) are predominant in swamp buffalo. This deletion was not found in
gayal, yak and cattle, though these all have another deletion in the intron of INHA, c. 262+78_262+79delTG. The results
of sequence alignment showed that the substitutions c. 43A and c. 67G in exon 1 of INHA are specific to buffalo, whereas
the substitutions c. 173A and c. 255G are exclusive to gayal, yak and cattle, and c. 24C, c. 47G, c. 174T and c. 206T are
specific to goat. Furthermore, there are few differences among gayal, yak and cattle, but there relatively great differences
between buffalo, goat and other bovine species regarding the sequences of INHA exon 1.
Keywords
Buffalo; Gayal; Yak; Inhibin α gene(INHA)exon 1; Polymorphism
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