In this study, both long PCR and conserved primers walking sequencing methods were used to determine the complete sequence of the of
Pyrgilauda ruficollis
mitochondrial genome (KC836121). The results showed that the complete mitochondrial genome of
P. ruficollis is 1 6909 bp in length with 55.0% A+T content, harboring the typical 37 genes. The mitogenome had the same gene order with that of
Podoces hendersoni
All protein coding genes started with ATG codon, except
ND3 with GTG. For the stop codon usage, most genes terminate with codons TAA or TAG, but
ND5 terminated with AGA, while
ND1 and
COI genes with AGG, and both the genes
COIII and
ND4 have an incomplete termination codon (T). The secondary structures of 22 tRNA genes were also predicted, showing that all tRNAs can form typical clover-leaf secondary structures, except for the
tRNASer (AGN) which loses the DHU arm, while
tRNAPhe harbor an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12
S rRNA and 16
S rRNA exhibit 47 helices in 4 domains and 60 helices in 6 domains respectively. The control region of
P. ruficollis with the length of 1 305 bp was located between
tRNAGlu and
tRNAPhe, and typical domains of which could be found as other bird groups. Using the data from 13 mitochondrial protein-coding genes, results of a final phylogenetic analysis strongly supports the traditional view that
P. ruficollis is closely related with Passeridae and Fringillidae.
