African Health Sciences Makerere University Medical School ISSN: 1680-6905 EISSN: 1729-0503 Vol. 7, Num. 3, 2007, pp. 155-158

African Health Sciences, Vol. 7, No. 3, June, 2007, pp. 155-158

Antibacterial activity of Thonningia sanguinea against some multi- drug resistant strains of Salmonella enterica

N’guessan J D¹; Coulibaly A¹; Ramanou A A¹; Okou O C^1,2 ; Djaman A J^1,3; Guédé-Guina F¹

¹Laboratoire de Pharmacodynamie Biochimique UFR Biosciences Université de Cocody-Abidjan
²Laboratoire de Bactériologie et Virologie-Institut Pasteur de Côte d’Ivoire
³Laboratoire de Biochimie Clinique et Fondamentale- Institut Pasteur de Côte d’Ivoire

* Corresponding author: N’guessan Jean David Maître-Assistant au Laboratoire de Pharmacodynamie Biochimique UFR Biosciences-Université de Cocody Abidjan 22 BP 582 Abidjan 22 e-mail: nguess100jd@yahoo.fr Tel : (00225) 05-78-57-89

Code Number: hs07031

Abstract

Background : The emergence of strains of S.enterica with multiple drug resistance (MDR) is of great concern worldwide.The extracts of flowers of Thonningia sanguinea are used in traditional medicine in Ivory Coast to treat diarrhoeal diseases including salmonellosis. Previous studies had shown inhibition of the MDR strain Salmonella Enteritidis lysotype 6.
Objectives: The present study focused to investigate the effect of the extract of the flowers of Thonningia sanguinea on some clinical MDR strains of Salmonella namely S.Tyhpi, S.Typhimurium,S. Hadar and a sensitive strain (S.Enteritidis).
Methods: The antimicrobial parameters were determined by double dilution with agar slant method.This method led us to determine MIC, IC ₅₀ and MBC.
Results: The MDR strain of S.Typhimurium presented the highest MIC (2.5 mg/ml) whereas the other two MDR strains (S.Hadar,S.Typhi) and the sensitive one (S.Enteritidis) had the same MIC (1.25 mg/ml).The four strains presented the same MBC (2.5 mg/ml). The MDR strain of S.Typhi is the most susceptible strain to the aqueous extract of the flowers of Thonningia sanguinea according to The IC₅₀values.
Conclusions: The aqueous extract of Thonningia sanguinea can provide an alternative therapy for the treatment of salmonellosis, mainly for typhoid fever caused by MDR strains of STyphi.The extract also inhibits S.Hadar a MDR emerging strain in Ivory Coast.

Key-words: Thonningia sanguinea; Salmonella, MDR strains, Ivory Coast African Health Sciences 2007; 7(3):

Introduction

Salmonella spp. are considered one of the major foodborne pathogens ¹ , resulting in considerable morbidity and occasionally death ² . It is a public-health problem in developing countries ³ . Salmonella enterica serovar typhi causes approximatively 10 million cases each year mostly in developing countries ^{4, 5} . Most of these infections have been attributed to consumption of poultry meat and eggs ⁶.

The emergence of strains of S. enterica with multiple drug resistance is of great concern worldwide. Increasing rates of antibiotic resistance have been reported in various regions throughout the world and certain antibiotic resistant clones, such as S. Typhimurium DT 104, seem to have spread in areas rather distant from each other ⁷.

In Ivory Coast, the typhoid fever and the other forms of salmonellosis became these last years a problem of public health taking into account the degradation of the conditions of healthiness in relation to the war. Ouattara ⁸have put in evidence the emergence of MDR strains of Salmonella belonging mainly to the serovar Hadar. The pathogenic role of Salmonella infection in the development of human diseases and the impact of resistance on the clinical outcome stimulated the search for newer treatments and natural products could provide alternative therapies against salmonellosis.

Thonningia sanguinea is used in traditional medicine for the treatment of haemorrhoids and anal lesions, bronchial asthma ^{9, 10, 11} , skin diseases, dysentery, sore throat and as vermifuge ¹² . In Ivory Coast, the flowers of Thonningia sanguinea are used for the treatment of diarrhoea ^{13, 14} which is known to be one of the symptoms of salmonellosis.

Previous studies have shown inhibition of the MDR strain Salmonella enteritidis lysotype 6 by the crude aqueous extract of Thonningia sanguinea ^{15, 16} . The antibacterial activity of T.sanguinea have also been studied by Ohiri and Uzodinma ¹⁷ . Two ellagitannins (Thonningianins A and B) with strong antioxidant activities have been isolated from Thonningia sanguinea ¹⁸.

In this study, as a part of our contribution in the fight against salmonellosis, we have evaluated the anti-microbial effects of the aqueous extract of T. sanguinea on three clinical MDR strains of Salmonella including S. Hadar and a sensitive one.

Material and methods

Plant material: Thonningia sanguinea is a parasitic plant and the flowers are the main part of the plant.The flowers of T.sanguinea were collected in Adzopé, Ivory Coast and identified by Pr Aké-Assi of the Department of Botany, University of Cocody-Abidjan.A voucher specimen (Voucher n° 14162) is deposited in the herbarium of “Centre National de Floristique” of Abidjan.

Extraction: The freshly collected flowers of the plant were air dried at room temperature for 7 days and powdered. Briefly 20g of powder was soaked in 500ml distilled water for 24 h with constant stirring.The suspension was further filtered through Whatmann (N°1) filter paper.The filtrate was concentrated in vacuo using a rotary evaporator to obtain the aqueous extract.

Bacterial strains: Bacteria for testing purposes were kindly provided by the Laboratory of Bacteriology and Virology of the Pasteur Institute of Ivory Coast. It was in-house reference strains.

• Salmonella typhi (330) resistant to ceftazidim, amoxicilin and clavulanic acid;
• Salmonella typhimurium (n° 4, 5, 12, i : 1, 2 12/02) resistant to Ceftazidim and Trimethoprim
• Salmonella hadar n°86.3 (337) resistant to Amoxicilin,Trimethoprim and Ceftazidim
• Salmonella Enteritidis (n°9,12 : g, m :-12/02) sensitive

Antibacterial tests

Determination of MIC and IC ₅₀

The Minimal Inhibitory Concentration (MIC) was de termined by the agar slant method ¹⁹.A 2-fold serial dilution (0–5000 µg/ml) of the crude extract in Mueller Hinton agar had been prepared in slant tubes.An aliquot of 10 µl of standardized suspension of bacteria (10³ cells/ ml) were added to each tube and incubated at 37 °C for 24 h. The lowest concentration of the tubes which did not show any visible growth after macroscopic evaluation was considered as the MIC. IC₅₀ defined as the drug concentration that produces 50% of the maximal effect [19] was determined as following. After one day (24 h) of incubation at 37ºC for 24 h, the colonies were then counted for each tube. The dose response curve was established in term of percentage of survivors:

Where

n = number of UFC counted in experimental tube
N = number of UFC in control tube

The test had been conducted in triplicate.

Determination of MBC

The Minimal Bactericidal Concentration (MBC) is defined as the concentration producing a 99.9 % reduction of colony number in the initial inoculum. It was determined by subculture on nutrient agar as previously described¹⁹ .After the MIC determination, the tubes without growth (concentration = MIC) were sub-cultured on Mueller Hinton agar in Petri dishes at 37°C for 24 h. MBC was defined as the lowest concentration of the sub-cultured tubes which did not show any visible growth after macroscopic evaluation. All the antibacterial parameters have been determined after triplicate assays.

Results

The extract exhibited some degree of antimicrobial action on the three MDR strains.

The MDR strain of S.typhimurium presented the highest MIC value (2.5 mg/ml).The MDR strains of S.Typhi, S.hadar and the sensitive strain of S.enteritidis presented the same MIC (1.25 mg/ml) (Table 1). All the tested strains had the same value of MBC (2.5 mg/ ml).

MIC and MBC led to the calculation of MBC/ MIC (Table 1) and this value was equal to 1 for the MDR strain of S.typhimurium and 2 for the other MDR strains. These values (MBC/MIC) indicated that the aqueous extract of the flowers of Thonningia sanguinea showed a bactericidal action on all the four tested strains.

The dose-response curve (Figure 1) led to the determination of the IC₅₀ (Table 2).The IC₅₀ values for S.typhi, S.Hadar, S.typhimurium were respectively 0.10 mg/ml, 0.45 ml and 1.25 mg/ml. According to the values of IC₅₀, between the MDR strains, S.Typhi was the most susceptible to the extract and S.Typhimurium is the less susceptible one.

The comparison of the antimicrobials parameters of the MDR strains with those of the sensitive strain showed that they had the same MBC (2.5 mg/ml). S. Typhi and S. Hadar possessed the same MIC (1.25 mg/ ml) with the sensitive strain. The IC₅₀ of the sensitive strain is higher than the IC ₅₀ of S.Typhi but it is smaller than the IC₅₀ of the others MDR strains. S.typhi is most susceptible to the extract than all the others tested strains including the sensitive one.

In this study, the aqueous extract of T.sanguinea was tested against one sensitive strain namely Salmonella Enteritidis and three MDR strains (S.Typhi, S.Hadar and S.Typhimurium).The red colour of the aqueous extract of Thonningia sanguinea in solution created interferences with the turbidity so it was difficult to determine with certainty the MIC by the recommended broth dilution method.This technical difficulty led us to use an alternative method (agar slant method) to evaluate the antimicrobial parameters of this kind of extract. Our results showed bactericidal effect of the aqueous extract of Thonningia sanguinea for all the MDR and sensitive tested strains. Moreover, S.Typhi (MDR), S.Hadar (MDR) and S.Enteritidis (sensitive) presented the same susceptibility to the aqueous extract of Thonningia sanguinea. These results indicate that the aqueous extract of Thonningia sanguinea contains some substances having target sites other than those used by antibiotics at which these strains are resistant. The inhibition of The MDR strains of S.Typhi responsible of Typhoid fever and S. Hadar which is an emerging MDR strains in Ivory Coast⁸ is a promising result for the treatment of salmonellosis induced by these MDR strains.

The antimicrobial activities of the flowers of T.sanguinea have already been reported by M’baïasbé¹⁵ . Using the disk diffusion method to evaluate the antimicrobial activity of the aqueous extract of T.sanguinea on the growth of S.Enteritidis, this author found IC₅₀ value of 4.68 mg/ml. Ouattara et al¹⁶. using the broth dilution method (recommended method for the antibacterial assays) have also tested the antimicrobial effect of this extract on Salmonella Enteritidis.They reported MIC and IC₅₀ values respectively 1.25 mg/ml and 0.189 mg/ml. Using in this study the agar slant method, our results gave the same MIC on S.Enteritidis with those of Ouattara et al.¹⁶and the IC ₅₀ values are similar. It is possible to say that the agar slant method can be also used as an alternative method to evaluate the antibacterial activity of coloured extracts.

The phytochemical screening of the extract of the flowers of Thonningia sanguinea have shown the presence of saponins, quinons, polyphenols ²⁰. These three classes of secondary metabolites are known to possess antibacterial activities²¹ and they may be responsible of the antimicrobial activity of the flowers of T.sanguinea.

The results of this study suggest that Thonningia sanguinea can provide alternative solution for the treatment of salmonellosis particularly in Ivory Coast where this pathology is always a public health problem⁸ .

Acknowledgment

We wish to express our gratitude to Dr Kra Adou Koffi Mattieu for his technical help.

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