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On Simulium (Pternaspatha), with Description of a New Species (Diptera-Simuliidae) S Coscaron/+, CL Coscaron-Arias* Facultad de Ciencias Naturales y Museo, 1900, La Plata, Argentina *Facultad de Ciencias Agrarias, 8324 Cinco Saltos, Rio Negro, Argentina
+Corresponding author. Fax: +54-21-257527 Simulium (Pternaspatha) diamantinum n. sp. is described from females, males, pupae and larvae, collected at Mendoza, San Juan and Neuquen. Some specimens of this species from Laguna del Diamante were included before by Wygodzinsky and Coscaron (1967) in S. strigidorsum Enderlein. The new species is compared with closely related species of the S. (Pternaspatha) "nigristrigatum group". The following synonym is established: Simulium (Pternaspatha) bachmanni Wygodzinsky & Coscaron = Simulium (Pternaspatha) nigristrigatum (Enderlein). Key words: southern Andean blackflies - description - new species - new synonym
Simulium (Pternaspatha) is a peculiar Neotropical subgenus composed of 26 species characteristic of the S. W. of South America on both sides of the Andes Cordillera from the Puna plateau to Patagonia, with one species in the northern Andes in Ecuador. This subgenus is characterized particularly by the following combination of characters: gray, pollinose females with variable ornamentation on the abdomen; a subquadrate, distally concave, smooth or rugose male distimere; pupa with a high number of trichomes variable in number, length and morphology (filiform to flattened), single spatulate to multirramose; and larva with dark head with mostly negative spots on the cephalic apotome and a deep, angular gular cleft. Revisionary works on the taxonomy of this subgenus were made by Wygodzinsky and Coscaron (1967), and Coscaron and Wygodzinsky (1972). More recently Wygodzinsky and Coscaron (1979), and Coscaron and Matta (1982) included two new species. Despite the taxonomic studies above there remained some doubts on species identity, mostly because not all stages (especially the pupa) had been described. An example is S. strigidorsum Enderlein, known only from type material (2EE) from Juliaca, Puno (Peru). Wygodzinsky and Coscaron (1967) included in this species females from Argentina, that show some differences with specimens from Peru. Another example is S. (Pternaspatha) dureti from San Juan, described from a single pharate male, a pupa and several larvae (Coscaron & Wygodzinsky 1972). From the present study based on females, pupae and larvae from Laguna del Diamante where S. (P.) strigidorsum was recorded by Wygodzinsky and Coscaron (1967), and specimens from Neuquen, we conclude that this material is not S. strigidorsum, and corresponds to a new species close to S. dureti, S. horcochuspi, S. deagostinii and S. pichi The previous incompletely described S. (P.) nigristrigatum (Enderlein 1930), is compared with S. (P.) bachmanni Wygodzinsky & Coscaron (1967). MATERIALS AND METHODS Material was collected in the breeding places, and pupae were maintained alive to obtain imagos. The collecting and dissection methodology used was that of Coscaron (1981). Type specimens of S. (Pternaspatha) strigidorsum (female lectotype), and S. (P.) nigristrigatum Enderlein (male lectotype, female syntype) from the Zoologisches Museum of Humboldt University, Berlin were examined. RESULTS Examination of the newly collected material, revision of previously collected specimens and their comparison with type material of S. strigidorsum permits us to establish a new taxon that is here described. Simulium (Pternaspatha) diamantinum n. sp. Female: wing length 3.3-3.7 mm. General color gray pollinose. Antennae, maxillary palp and proboscis blackish. Head brown, dark gray pollinose. Frons, clypeus and occiput blackish with gray pollinosity; occiput a little darker with hairs grayish to black in accordance with the light. Mesonotum grayish black with a silvery-gray, submedian, lyre-shaped pattern, lighter anteriorly and grayish laterally and posteriorly (Fig. 1); with changing light position the lyre- shaped pattern becomes thicker, and in some lights its anterior border becomes black, to a silky bright light gray. Mesonotum hairs sparse and upright. Scutellum blackish-gray. Scutum and scutellum hairs light gray. Metanotum velvet blackish. Pleura blackish gray. Wings with yellowish veins and brown hairs and spines. Stem of halteres yellowish-brown, the knob yellowish-white. Legs blackish with basal third of femur III, basal half of tibiae I-III, basal half of basitarsus II and 3/4 of basitarsus III and base of tarsites II-III light brownish. Distribution of pigmentation of hind leg as Fig. 3. Abdomen light grayish pollinose, tergite I dark grayish; black subtriangular median spots on tergites II-V, lighter and pollinose on VII, and 1+1 lateral, blackish, subrectangular spots not reaching posterior border on tergites IV-V (Figs 4- 5). Hairs of pleural tuft light gray. Frons as Fig. 6. Fronto- ocular triangle about as long as wide (Fig. 7). Sensory vesicle of palp about 1/3-1/4 of basal articles, its structure as in Fig. 8. Antenna and cibarium as S. dureti, typical of the subgenus Pternaspatha. Mandible with 25+8-30+9 teeth, and maxilla 23-25 teeth. Sc with 3-5 setae. Base of R without setae; R1 and Rs with setae and spines arranged in one irregular row. Shape and proportion of articles of leg III as shown in Fig. 2. Without calcipala; ratio of length to width of posterior basitarsus = 7.2-7.6 (Fig. 3). Claws with well developed basal tooth (Fig. 9). Eighth sternite well sclerotized, with 34-40 hairs on each side, gonapophysis subtriangular (Fig. 10). Paraproct and cercus as in Fig. 11. Genital fork as in Fig. 12; spermatheca with small internal spines. Male: wing length 2.8-3.0 mm. Head coloration as in female. Scutum black-grey with 1+1 subtrian-gular spots anteriorly (Fig. 13); with light anterior to specimen 1+1 anterior gray pollinose spots occupying anterior third. Decumbent, silky grayish hairs sparsely covering scutum. Abdomen blackish with 1+1 large gray spot on tergites II, smaller on tergites VII-VIII, VI and IX mostly grayish except for a small and short median darker spot. Pleura, halteres and wings as female. Legs yellowish brown with light gray hairs; blackish brown on coxae, apex of femur III, apex of tibiae, all of basitarsus I, distal third of basitarsi II and III and tarsites. Hind basitarsus three times as long as wide; calcipala absent (Fig. 14). Genitalia very close to species of S. (Pternaspatha) "nigristrigatum group"; distimere with abundant tubercles (Fig. 15). Pupa: cocoon shoe-shaped with well developed antero- ventral bridge (Fig. 16); surface generally smooth, but some specimens with small sand granules incorporated. Cocoon closely woven. Respiratory organs covered basally by walls of cocoon. Length of cocoon 3.8-4.0 mm at dorsum, maximum length along base 5.2-6.0 mm, length of body 4.0-4.7 mm, of gills 2.2-3.3 mm. Respiratory organ with eight filaments, position of basal branching as Fig. 17. Frontoclypeus and thorax with 500-1000 platelets (Fig. 18) those of thorax slightly pointed, especially on the posterior border (Fig. 19), others rounded with a smooth (Fig. 20) or slightly rough surface (Fig. 21); trichomes filiform with 4-8 branches, 5-15 frontal and 3-7 facial trichomes on each side in a continuous group. Length of frontal trichomes 0.19-0.23 mm; thoracic trichomes 0.24- 0.35 mm in number of 160-220, with 6-10 filiform branches (Fig. 22). Chaetotaxy of abdomen following the general pattern of S. (Pternaspatha) species. Larva: maximum length 7.5-8.5 mm. Head width 0.7 mm. Color dark, greenish brown, ornamentation and shape in accordance to Fig. 23. Integument smooth. Cephalic apotome brown with negative spots without well defined borders (Fig. 24). Antennae dark brown; ratio of length of segments I-III = 1:1.8-1.9:1.2-1.3 (Fig. 25). Cephalic fans with 35-39 combs. Rays of internal border of combs of variable length 3-5 short rays alternating with one longer ray. Mandible with 4-6 secondary teeth arranged in several rows (Figs 26-27). Maxillary palp with 4-5 papillae distally. Teeth of hypostomium border as Fig. 28; 5-6 lateral serrations. 15-17 lateral setae arranged in 1-2 rows; disc of hypostomium with 3-5 dispersed setae. Hypostomial bridge short, ratio hypostomium/hypostomial bridge = 1.4-1.5. Comb of pseudopod with about 43-54 teeth arranged in 12-14 groups (Fig. 29). Anal sclerite without scales but with isolated trichomes as in Fig. 30. Crochet ring with about 94-100 rows, each composed of about 15-17 hooks. Anal gills with 5-7 lobules on each of the three well developed lobes (Fig. 31). Material examined: Argentina, Mendoza, Laguna del Diamante, 3,350-3,400 m, 27-I-95, coll. Coscaron 1 female holotype; 77 female paratypes; same locality 3,380 m, 27-I-55, coll. Aczel 10 female paratypes; same locality, rio Diamante 3,300 m 27-I-95: 4 pupae, 3 larvae in late stage, 8 immature larvae; Neuquen Route 40, 3 km South of Buta Ranquil, 1,200 m 22-IX-75, Coscaron: 1 male allotype 3 female and 5 male reared, 30 larvae, 20 pupae; San Juan, Tocota. 2,400 m one pharate male, one pupa previously identified as S. (P.) dureti. Type material in Museo La Plata. One female cited by Coscaron and Wygodzinsky (1972) as S. strigidorsum from Argentina: Chubut, Alto rio Senguerr, l2-II-64, Bejarano, in the Instituto Nacional de Microbiologia (Buenos Aires) has been lost. Bionomics: females are anthropophilic and a biting pest in Laguna del Diamante area, a tourist resort frequented by fisherman. Larvae and pupae were collected on Potamogeton at a short distance from the breeding place in the Diamante river. At the collection site, the river is clear with slow current. At San Juan (Tocota), the breeding ground was in a small creek with a strong current and in northern Neuquen in a medium sized creek. DISCUSSION After re-examination of the female lectotype of S. (P.) strigidorsum Enderlein and comparison with new material from Laguna del Diamante the following differences are shown in Table I. Male, pupa and larva of S. strigidorsum are unknown. There are also similarities and differences between S. diamantinum n. sp. and some other species close to Pternaspatha "nigristrigatum group" (Table II). Data on Table II show that pichi, dureti, diamantinum, horcochuspi and deagostinii are very closely related species which can be distinguished by some morphological characters and different distributions. S. diamantinum can be distinguished from the other species by several distinctive characters such as: female abdomen with 1+1 small, narrow and short, black lateral spots on tergites III-V, well isolated from the tergites median black spot and leaving a wide white transverse band before the posterior border, scutum hairs not adpressed, narrower posterior basitarsi, longer pupa gill, higher number of frontal and thorax trichomes, longer thoracic trichomes, and in the larva a longer second antennal segment, more lateral setae on the hypostomium, and a shorter hypostomial bridge in relation to hypostomial length. Another Pternaspatha species that requires further study is S. nigristrigatum Enderlein particularly the currently unknown immature stages. This species is sympatric with S. (Pt.) bachmanni Wygodzinsky & Coscaron. S. nigristrigatum was originally described from one male and five females from Neuquen, (Neuquen Province, Argentina). Later, Wygodzinsky and Coscaron (1967) studied more material from localities at near Neuquen, where bachmanni is also present. S. bachmanni was described in the female, male, pupal and larval stages from a locality 40 km east of the nigristrigatum type locality in the Negro river, formed by the confluence of the Neuquen and Limay rivers. Posteriorly has been collected in the Limay river always at the same locality (Coscaron & Wygodzinsky 1972). S. bachmanni was considered by Wygodzinsky and Coscaron (1967) as a new species based mainly on ornamental and morphological differences with nigristrigatum female. S. bachmanni is a very well known species with a distribution on Rio Negro High and Medium Valleys, Colorado, Chubut and Tecka rivers at different localities (Coscaron 1991). It is a distinctive species in the immature stages, particularly the morphological characters of the pupa such us the absence of platelets on the thorax and frontoclypeus and the sparse thoracic trichomes of which there are about 10 [in other Simulium (Pternaspatha) species always are more than 10]. Ornamental differences that differenciate bachmanni and nigristrigatum occur particularly on the female abdomen, which has 1+1 sub-lateral, whitish spots as is shown in Wygodzinsky (1958, Fig. 21), with a curved anterior border, or in Wygodzinsky and Coscaron (1967) and Coscaron (1991) showing a more acute anterior border, as also occurs in the nigristrigatum female type specimen revised recently. Simulium bachmanni also shows similar intraspecific variations in abdominal ornamentation (Wygodzinsky & Coscaron 1967, Coscaron 1991) but the more common condition is shown in Fig. 35. The male nigristrigatum lectotype (Stone 1962) does not show morphological and ornamental differences with S. bachmanni. Male basitarsi proportions and morphology of the genitalia of S. nigristrigatum (Fig. 36) are similar to those seen in S. bachmanni. Previous collections in the Limay, Neuquen and Negro rivers, and other Patagonian creeks, produced immature stages indistinguishable from S. bachmanni. Based on this evidence we consider S. (Pternaspatha) bachmanni Wygodzinsky & Coscaron 1967 to be a junior synonym of S. (Pternaspatha) nigristrigatum Enderlein 1930. AKNOWLEDGEMENTS To Dr H Schumann from the Zoologisches Museum of the Humboldt University, Berlin, for the loan of type material, to Escribano Benito Perez from Comision Mendoza del Valle de Uspallata for help in transporting the authors to Laguna del Diamante, and to Prof. Nelida Caligaris for the illustrations. REFERENCES Coscaron S 1981. Insecta, Diptera, Simuliidae: 105 pp., 15 pl., fotos. In RA Ringuelet. Fauna de Agua Dulce de la Republica Argentina 38. Fasc. 1. Coscaron S 1991. Insecta, Diptera, Simuliidae: 304 pp., 67 pl., In ZA de Castellanos. Fauna de Agua Dulce de la Republica Argentina 38. Fasc. 2. Coscaron S, Matta A 1982. Notas sobre Simulidos Neotropicales X. Sobre dos nuevas especies de "jerjeles" del genero Simulium Latreille del Norte de Chile (Simuliidae, Diptera, Insecta). IDESIA (Chile) 6: 49-61, figs. Coscaron S, Wygodzinsky P 1972. Taxonomy and distribution of the Black Fly subgenus Simulium (Pternaspatha) Enderlein (Simuliidae, Diptera, Insecta). Bull Amer Mus Nat Hist 147: 201-240, figs. Enderlein G 1930. Der heutige Stand der Klassifikation der Simuliiden. Arch Klassif Phylog Ent 1: 77-97. Stone A 1962. Notes on the types of some Simuliidae (Diptera) described by Enderlein. Ann Ent Soc America 55: 206- 209. Wygodzinsky P 1958. Notas y descripciones de Simuliidae patagonicos y andinos (Diptera). Acta Zool Lilloana 16: 121-148. Wygodzinsky P, Coscaron S 1967. A review of Simulium (Pternaspatha) Enderlein (Simuliidae, Diptera). Bull Am Mus Nat Hist 136: 1-116. Wygodzinsky P, Coscaron S 1979. Description of Black Fly of the subgenus Simulium (Pternaspatha) from the High Andes of Ecuador (Simuliidae, Diptera). Amer Mus Novitates 2670: 1-9, figs.
Figures 16-31: Simulium diamantinum - Figs 16-22: pupa. Fig. 16: general aspect. Fig. 17: respiratory filaments. Fig. 18: frontoclypeus. Figs 19-21: thorax platelets. Fig. 22: thorax trichome. Figs 23-31: larva. Fig. 23: general aspect. Fig. 24: cephalic apotome. Fig. 25: antenna. Figs 26-27: dentary portion of mandible. Fig. 28: hypostomium and angular cleft. Fig. 29: pseudopody comb. Fig. 30: anal sclerite. Fig. 31: anal gills. Figures 32-36: Simulium strigidorsum, female. Fig. 32: abdomen in dorsal view. Fig. 33: hind leg. S. horcochuspi. Fig. 34: pupa, general aspect. S. bachmanni. Fig. 35: abdomen of female, dorsal view. S. nigristrigatum lectotype male. Fig. 36: posterior basitarsus. TABLE I: Differences between Simulium strigidorsum (Juliaca) and S. diamantinum (Laguna del Diamante)
strigidorsum diamantinum n. sp. Shorter, wing length: Longer, wing length: 3.1-3.2 mm 3.4-3.9 mm TABLE II: Characters of Simulium pichi, S. dureti, S. diamantinum, S. horcochuspi and S. deagostinii
Species pichi dureti
-----------------------------------------------------
FEMALE
Scutum hairs:adpressed + +
dense + +
bright color silvery silvery
1+1 lateral black spots on T.III-V
reaching posterior border + +
Wing length 2.4-2.9 3.2-3.7
Calcipala small small
Claws subbasal tooth short short
Ratio length/width
posterior basitarsus 6.4 6.7
MALE
Ratio length/width
posterior basitarsus 3.8 3.2-3.4
PUPA
Cocoon slipper- shoe-
shaped, shaped,
light large
brown antero-
thin ventral
trans- bridge,
lucent thick
trans-
lucent
Maximum gill length 1.2-1.6 2.0-2.5
No. of frontoclypeus platelets 600-900 600-800
No. of frontal trichomes
on each side 3 12
No. of facial trichomes
on each side 1-2 4
No. of thorax trichomes
on each side 60 150
Trichomes length 0.08-0.12 0.10-0.19
LARVA
Maximum length 6.7 8.5
Cephalic apotome spots light brown, dark brown,
positive negative l
pattern
concolorous
with head
apotome
Proportion of antennal
articles I-III 1:1.1:0.9 1:1.3-1.4:
0.7-0.8
No. of mandibular
secondary teeth 6-8 3-4
Lateral setae of
hypostomium 11 11-14
Ratio length of hypos-
tomium/hypostomial bridge 1/1.1 1/3
No. of rows in the
crochet ring 83-92 84-92
No. of hooks 18 17
No. of anal gill lobules on
each lobe 0 0
No. of teeth in the
pseudopod comb 42 34
Habitat altitude (m) 10-200 1,800-4,300
Provinces Chubut, Jujuy, Salta
Santa Cruz
Endemism area Patagonia Puna
Table II continued
Species diamantinum horcochuspi
------------------------------------------------------------
FEMALE
Scutum hairs:adpressed - +
dense - -
bright color silvery silvery
1+1 lateral black spots on T.III-V
reaching posterior border - +
Wing length 3.4-3.9 2.9-3.6
Calcipala absent absent
Claws subbasal tooth large short
Ratio length/width
posterior basitarsus 7.2-7.6 5.8-6.0
MALE
Ratio length/width
posterior basitarsus 3.3 3.8
PUPA
Cocoon shoe- slipper-
shaped, shaped,
large brownish,
antero- gray
ventral leaden
bridge, sheen,very
thick thin not
trans- translucent
lucent (Fig. 34)
Maximum gill length 2.2-3.0 1.8-2.3
No. of frontoclypeus platelets 500-1000 400
No. of frontal trichomes
on each side 5-15 3-4
No. of facial trichomes
on each side 3-6 1
No. of thorax trichomes
on each side 160-220 60-85
Trichomes length 0.24-0.35 0.25
LARVA
Maximum length 8.5 8.0
Cephalic apotome spots light brown, light brown,
negative not negative not
well defined well defined
Proportion of antennal
articles I-III 1:1.8-1.9: 1:1.6-1.7:
1.2 0.8-1.0
No. of mandibular
secondary teeth 4-6 6
Lateral setae of
hypostomium 15-17 13-14
Ratio length of hypos-
tomium/hypostomial bridge 1/4-1/5 1/2
No. of rows in the
crochet ring 94-100 110
No. of hooks 15-17 22
No. of anal gill lobules on
each lobe 5-7 0
No. of teeth in the
pseudopod comb 43-54 38
Habitat altitude (m) 1,200-3,400 3,900-4,200
Provinces San Juan, Jujuy
Mendoza,
Neuquen
Endemism area Puna, Puna
Patagonia
Table II continued
Species deagostinii
-----------------------------------------------
FEMALE
Scutum hairs:adpressed +/-
dense +/-
bright color tin
1+1 lateral black spots on T.III-V
reaching posterior border +
Wing length 3.3-3.5
Calcipala absent
Claws subbasal tooth large
Ratio length/width
posterior basitarsus 6.6
MALE
Ratio length/width
posterior basitarsus 3.7
PUPA
Cocoon shoe-shaped,
large
antero-ventral
bridge,
translucent
Maximum gill length 2.0-2.4
No. of frontoclypeus platelets 600-800
No. of frontal trichomes
on each side 4-6
No. of facial trichomes
on each side 3
No. of thorax trichomes
on each side 35-80
Trichomes length 0.17-0.18
LARVA
Maximum length 8.0
Cephalic apotome spots dark brown,
negative well
defined
Proportion of antennal
articles I-III 1:1.3-1.5:0.8-1.0
No. of mandibular
secondary teeth 4-5
Lateral setae of
hypostomium 14-15
Ratio length of hypos-
tomium/hypostomial bridge 1/3
No. of rows in the
crochet ring 95-98
No. of hooks 17-18
No. of anal gill lobules on
each lobe 4-6
No. of teeth in the
pseudopod comb 37
Habitat altitude (m) 10-300
Provinces Santa Cruz,
Tierra del Fuego
Endemism area Subantarctic
Copyright 1996 Fundacao Oswaldo Cruz
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