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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 19, No. 2, 2007, pp. 43-48
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Bioline Code: bk07007
Full paper language: English
Document type: Research Article
Document available free of charge
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Biokemistri, Vol. 19, No. 2, 2007, pp. 43-48
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Cofactor Interactions In The Activation Of Tissue Non-Specific Alkaline Phosphatase: Synergistic Effects Of Zn2+ And Mg2+ Ions
Olorunniji, Femi J.; Igunnu, Adedoyin; Adebayo, Joseph O.; Arise, Rotimi O. & Malomo, Sylvia O.
Abstract
The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of p-nitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ionsin promoting the hydrolysis of p-nitrophenylphosphate by alkaline phosphatase. The results show that assays of alkaline phosphatase activity in homogenised tissue samples will give better responses if both Mg2+ and Zn2+ ions are included in the reactions
Keywords
Alkaline phosphatase, kinetics, enzyme-cofactor interaction, synergism
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© Copyright 2007 - Nigerian Society for Experimental Biology
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