The plant micro-propagation in bioreactor systems is regarded as one way to reduce cost by automation
and production scheduling. This research was carried out in order to obtain an efficient procedure for clone
production of
Eucalyptus camaldulensis
on different types of bioreactor including continuous and temporary
immersion bioreactor. To do so, the apical meristems (1 mm) and the apical meristems with adjacent tissue
(2,5 mm) were used as initial explants. These tissues were cultured, for 60 days, in semisolid culture medium
supplemented with 1 mg L
-1 indole acetic acid (IAA) and 0.32 mg L
-1 benzylaminopurine (BA). After 60 days,
the meristems with adjacent tissue were transferred to a continuous immersion bioreactor and maintained in
dark or light conditions. In order to verify the effect of the explant source on bioreactor multiplication, the
explants subcultured from meristems multiplied in semisolid culture medium and the meristems multiplied
in continuous immersion bioreactor were tested and maintained in dark conditions. After establishing this
parameters, the multiplication experiments were carried out in continuous and temporary immersion and
the multiplied explants were then rooted in MS medium supplemented with 0, 2, 4, 8 and 20 mg L
-1 indole
butyric acid (IBA) and kept in the dark or under controlled lighting conditions. After that, the rooting the
plants were acclimatized in mist chamber. The meristem with adjacent tissue favored a greater number
of buds/explants. The continuous immersion bioreactor in the dark provided higher shoots number and
multiplication rate. The rooting was better on culture medium without auxin and kept in the dark for 15 days
or the culture medium supplemented with auxin and maintained under light with 100% plantlet rooting. The
Eucalyptus camaldulensis acclimatization was efficient, with high survival rate (76%). It was possible to
establish the procedure for bioreactor micro-propagation of
Eucalyptus camaldulensis large-scale clones.