The aim of this study was to investigate the influence of arbuscular mycorrhizal fungi (AMF)
Glomus mosseae
(T.H.
Nicolson & Gerd.) Gerd. & Trappe and
Acaulospora laevis
(Gerd. & Trappe) on linseed (
Linum usitatissimum
L.) growth
response with phosphate solubilizing bacteria
Pseudomonas fluorescens
; different doses of superphosphate were used: 20
kg ha
-1 (half recommended dose), 40 kg ha
-1 (recommended dose), and 80 kg ha
-1 (double recommended dose) in earthen
pots filled with sterilized soil under greenhouse conditions. Among all the growth parameters, the following were the
highest in the
G. mosseae +
P. fluorescens combination at the medium concentration (recommended superphosphate dose):
plant height (78.74 ± 1.8 cm), fresh shoot weight (3.45 ± 0.294 g), dry shoot weight (0.57 ± 0.007 g), fresh root weight
(0.223 ± 0.023 g), dry root weight (0.036 ± 0.004 g), root length (17.67 ± 0.48 cm), AM spore number (94.4 ± 9.86),
shoot (1.14 ± 0.115%) and root (1.29 ± 0.110%) P content, and acidic (0.447 ± 0.012 IU g
-1 FW) and alkaline phosphatase
activity (0.119 ± 0.008 IU g
-1 FW). The percentage mycorrhizal root colonization with the
A. laevis +
P. fluorescens
(86.86 ± 2.17%) combination and chlorophyll content with the
G. mosseae +
A. laevis +
P. fluorescens (0.474 ± 0.009 mg
g
-1 FW) combination recorded the highest values at the low concentration (half recommended superphosphate dose) as
compared with non-mycorrhizal plants (control). The high superphosphate dose clearly reduced or decreased all the growth
parameters. Therefore, vigorous growth and maximum flax yield can be achieved by inoculating plants with AM fungi and
P. fluorescens with the recommended dose or less than the recommended dose of superphosphate.
