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3-Dichloroacetyl oxazolidine protect maize from imazethapyr herbicide injury
Zhao, Li-Xia; Wu, Hao; Fu, Ying; Zou, Yue-Li & Ye, Fei
Abstract
Safeners are an important tool used to ensure the safe using
of herbicide. The objective of this paper was to investigate
the protective effect of four 3-dichloroacetyl oxazolidine
safeners (3-dichloroacetyl-2,2-dimethyl-1,3-oxazolidine
[R-28725], racemate of 3-dichloroacetyl-2,2-dimethyl-4-
ethyl-1,3-oxazolidine, and its two chiral stereoisomers) in
reducing the injury caused by imazethapyr. Physiological
and biochemical tests were conducted under laboratory
condition, by using seed treatment with safeners and soil
treatment with imazethapyr, respectively. The interaction
of two safeners (R-28725 and R-stereoisomer) and
imazethapyr reduced the injury of maize significantly, and
also increased glutathione content, activity of glutathione
S-transferases, and activity of acetolactate synthase
in maize. When induced by R-stereoisomer, the GSH
content in root and in shoot increased 100.7% and 73.6%,
respectively. When induced by R-28725, the GST activity
in vivo increased threefold and the GST activity in vitro
more than doubled. The kinetic parameter Vmax of GST
in the maize treated with R-28725 and R-stereoisomer
increased by 102.2% and 81.9%, respectively, compared
with the control. The results also showed that R-28725 and
R-stereoisomer induced glutathione S-transferases affinity
for the substrate of conjugation reaction significantly.
Keywords
3-Dichloroacetyl oxazolidine; biological activity; GST activity; GST affinity; Zea mays
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