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Effects of brassinolide application on antioxidant characteristics and endogenous hormones of Leymus chinensis  (Trin.) Tzvelev under different light intensity regimes
Zong, Xue-feng; Yang, Ai-jie; Anjum, Shakeel Ahmad; Lv, Jun; Li, Na-jia; He, Xiu-juan; Xu, Yu; Wu, Xiao & Wang, San-gen
Abstract
Different light and shade intensities are the main constraints to achieve potential biomass accumulation in Leymus
chinensis (Trin.) Tzvelev. An experiment was conducted to explain the physiochemical and morphological adversity
triggered by various light intensities. Adverse impacts were lessened with exogenous brassinolide (BR). Treatments
consisted of 100% natural light (T0), 100% natural light + 0.1 mg L-1 BR (BT0), light shade (70% natural light) (T1), light
shade + BR (70% natural light + 0.1 mg L-1 BR) (BT1), moderate shade (50% natural light) (T2), and moderate shade +
BR (50% natural light + 0.1 mg L-1 BR) (BT2). These were completely randomized design treatments with five replicates.
Results showed that dry biomass production and endogenous hormone levels (abscisic acid [ABA], zeatin riboside [ZR],
gibberellic acid [GA], and indole acetic acid [IAA]) increased, while osmolyte accumulation (soluble sugars and proteins,
free amino acids, and proline), antioxidant activity (peroxidase [POD], superoxide dismutase [SOD], and catalase [CAT]),
and lipid peroxidation decreased under shade at T0. Treatment T1 depicted the most promising results for these attributes.
However, biomass accumulation (33%-56%), osmotic substances (soluble sugars,13%-38%; soluble proteins, 9%-41%;
free amino acids, 8%-16%; proline, 11%-41%), and antioxidant enzyme activities (POD, 11%-14%; SOD, 3%-33%; CAT,
4%-23%) increased. On the other hand, lipid peroxidation was reduced by BR application, as indicated by decreased
malondialdehyde (MDA) content (7%-31%) and relative conductivity (7%-19%) under both natural and shade conditions.
Likewise, the biosynthesis of ABA (2%-22%), ZR (11%-24%), and GA (30%-50%) improved under exogenous BR
compared with the control. The IAA contents (22%-28%) decreased under foliar-applied BR compared with the control.
Results suggested that light to moderate shade improved the biomass production and physiological performance of L.
chinensis, while BR application exhibited further improvement.
Keywords
Antioxidants; brassinolide; endogenous hormones; Leymus chinensis; shading
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