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Kinetic of Azo Dyes Decolourization by Enterobacteriaceae Species in the Intact Cell Assay System
Gondaliya, Shailesh B. & Pathak, Siddharth J.
Abstract
The method “Intact cell assay” was adopted to assess the influencing factors on the process of
decolourization ability of the enterobacteriaceaea specie isolates under predisposed environment. Taken into account
several ingredient are added individually as well in combinations in the assay reaction. Values of dye decolourized in μg
ml-1 with Multi vitanmins solution (MVS) with glucose 42.53; MVS 36.00; Riboflavin (RF) 39.00; Yeast extract 36.00
and B- complex 23.00 from the initial dye of 46.00μg ml-1 were observed , where as glucose, Ascorbic acid, Cysteine,
Cetyl-trimethyl-ammonium bromide (CTAB), Sodium molybdate, Biotin, KNO3, NaNO2, folic acid and 1-amino-2-
naphtho-4-sulfonic acid (ANSA) does not shows influence on to the process, but, some of them showed inhibitory
effect toward the decolourization. It was observed that the riboflavin addition at 19.95 n moles ml-1 in the reaction
mixture, rate of decolourization was suddenly change from 0.019 ΔA/minutes to 0.20 ΔA/minutes, which is extremely high
by 10 fold fast and subsequently remains faster i.e 0.2 ΔA/minutes without further additional RF in the same assay
mixture. Rate of decolourization with different concentrations of riboflavin i.e. 13.0 n moles ml-1 to 59.9 n moles ml-1
showed second order kinetic. This indicates that the minimum amount of RF is essential to trigger the process of
decolourization by the intact cells under the assay condition. While five different azo dyes were subjected, showed diverse
behavior on to the rate of decolourization. Results of entire study incite on role of riboflavin could to a certain extent act as
a redox mediator in the reaction(s) process and electron mediator between intracellular pool to the dye available at
periplasmic redox sink
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