![](/imgs/covers/jp_cover.jpg)
|
Journal of Postgraduate Medicine
Medknow Publications and Staff Society of Seth GS Medical College and KEM Hospital, Mumbai, India
ISSN: 0022-3859 EISSN: 0022-3859
Vol. 54, No. 3, 2008, pp. 181-185
|
Bioline Code: jp08064
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Journal of Postgraduate Medicine, Vol. 54, No. 3, 2008, pp. 181-185
en |
Comparison of a dipstick enzyme-linked immunosorbent assay with commercial assays for detection of Japanese encephalitis virus-specific IgM antibodies
Shrivastva, A; Tripathi, NK; Parida, M; Dash, PK; Jana, AM & Lakshmana, Rao PV
Abstract
Background: Japanese encephalitis (JE) is a major public health concern in Asia including India. Objectives: To evaluate an in-house developed dipstick enzyme-linked immunosorbent assay (ELISA) test vis-à-vis two commercial kits for detection of JE virus-specific IgM antibodies. Setting and Design: Comparative study carried out in Research and Development centre. Materials and Methods: A total of 136 specimens comprising 84 serum and 52 CSF samples were tested by in-house dipstick ELISA, Pan-Bio IgM capture ELISA (Pan-Bio, Australia) and JEV CheX IgM capture ELISA (XCyton, India). Results: The overall agreement among all three tests was found to be 92% with both serum and cerebrospinal fluid (CSF) samples. The sensitivity of the dipstick ELISA was found to be 91% with serum and 89% with CSF samples respectively. The specificity of the dipstick ELISA with reference to both commercial assays was found to be 100% in serum and CSF samples in this study. Conclusions: The in-house dipstick ELISA with its comparable sensitivity and specificity can be used as a promising test in field conditions since it is simple, rapid and requires no specialized equipment.
Keywords
Antibody, diagnostic test, dipstick enzyme-linked immunosorbent assay, Japanese encephalitis, virus
|
|
© Copyright 2008 Journal of Postgraduate Medicine. Alternative site location: http://www.jpgmonline.com
|
|