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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857 EISSN: 0255-0857
Vol. 28, No. 4, 2010, pp. 348-353
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Bioline Code: mb10103
Full paper language: English
Document type: Research Article
Document available free of charge
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Indian Journal of Medical Microbiology, Vol. 28, No. 4, 2010, pp. 348-353
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Utility of in vitro proton magnetic resonance spectroscopy in aetiological characterisation of brain abscesses
Menon, S.; Bharadwaj, R.; Chowdhary, A. S.; Kaundinya, D. V. & Palande, D. A.
Abstract
Purpose: Brain abscesses often present an aetiological dilemma. Microscopy is insensitive and culture techniques are time consuming. Hence, a new rapid technique in vitro Proton Magnetic Resonance Spectroscopy ( 1 HMRS) was evaluated for its usefulness in the identification of aetiology of brain abscesses.
Materials and Methods: A total of 39 pus specimens from brain abscesses were subjected to in vitro 1 HMRS. These pus specimens were also processed by conventional culture methods. The spectral patterns generated by in vitro 1 HMRS were further correlated with culture results.
Results: Pus specimens which showed the presence of anaerobes on culture revealed the presence of multiplet at 0.9 ppm (100%), lactate-lipid at 1.3 ppm (100%), acetate at 1.92 ppm (100%) and succinate at 2.4 ppm (75%). Pus specimens that revealed the presence of facultative anaerobes on culture showed a pattern B, i.e., the presence of lactate-lipid at 1.3 ppm (100%), acetate at 1.92 ppm (88.88%) along with the multiplet at 0.9 ppm (100%). Pattern C was seen in aerobic infection which showed the presence of lactate-lipid at 1.3 ppm (100%) along with the multiplet at 0.9 ppm. Pus from two tuberculous abscesses showed the complete absence of multiplet at 0.9 ppm.
Conclusions: We observed in this study that it was possible to differentiate bacterial and tuberculous brain abscesses using in vitro 1 HMRS. Further, it was also possible to distinguish between aerobic and anaerobic brain abscesses on the basis of spectral patterns. In vitro 1 HMRS of fungal and actinomycotic brain abscess are also presented for its unusual spectra.
Keywords
In vitro proton MR spectroscopy, pyogenic brain abscesses, tuberculous brain abscess
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© Copyright 2010 Indian Journal of Medical Microbiology. Alternative site location: http://www.ijmm.org
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