|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 6, 2016, pp. 391-398
|
Bioline Code: oc16057
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 6, 2016, pp. 391-398
en |
Pathogenicity and phenotypic sulfadiazine resistance of Toxoplasma gondii isolates obtained from livestock in northeastern Brazil
Oliveira, Claudio BS; Meurer, Ywlliane SR; Andrade, Joelma MA; Costa, Maria ESM; Andrade, Milena MC; Silva, Letícia A; Lanza, Daniel CF; Vítor, Ricardo WA & Andrade-Neto, Valter F
Abstract
Toxoplasma gondii is the causative protozoan agent of toxoplasmosis, which is a common infection that is widely
distributed worldwide. Studies revealed stronger clonal strains in North America and Europe and genetic diversity
in South American strains. Our study aimed to differentiate the pathogenicity and sulfadiazine resistance of three T. gondii isolates obtained from livestock intended for human consumption. The cytopathic effects of the T. gondii isolates
were evaluated. The pathogenicity was determined by polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) using a CS3 marker and in a rodent model in vivo. Phenotypic sulfadiazine resistance
was measured using a kinetic curve of drug activity in Swiss mice. IgM and IgG were measured by ELISA, and the
dihydropteroate synthase (DHPS) gene sequence was analysed. The cytopathic effects and the PCR-RFLP profiles
from chickens indicated a different infection source. The Ck3 isolate displayed more cytopathic effects in vitro than
the Ck2 and ME49 strains. Additionally, the Ck2 isolate induced a differential humoral immune response compared
to ME49. The Ck3 and Pg1 isolates, but not the Ck2 isolate, showed sulfadiazine resistance in the sensitivity assay.
We did not find any DHPS gene polymorphisms in the mouse samples. These atypical pathogenicity and sulfadiazine
resistance profiles were not previously reported and served as a warning to local health authorities.
Keywords
atypical profiles; pathogenicity; sulfadiazine-resistance; Toxoplasma gondii
|
|
© Copyright 2016 - Memórias do Instituto Oswaldo Cruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|