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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 11, 2016, pp. 686-691
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Bioline Code: oc16109
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 11, 2016, pp. 686-691
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Symbiont modulates expression of specific gene categories in Angomonas deanei
Penha, Luciana Loureiro; Hoffmann, Luísa; Souza, Silvanna Sant’Anna de; Martins, Allan Cézar de Azevedo; Bottaro, Thayane; Prosdocimi,, Francisco; Faffe, Débora Souza; Motta, Maria Cristina Machado; Ürményi, Turán Péter & Silva, Rosane
Abstract
Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and
some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as
respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this
paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes
of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed
that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential
gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment
analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved
in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the
upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic
process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei.
Keywords
trypanosomatid RNA-seq; gene expression; aposymbiotic strain; endosymbiosis
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