Purpose: To evaluate the antitumor activities of
Platycarya strobilacea
infructescence extracts in A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB cell lines.
Methods: The total amount of phenolics in
P. strobilacea infructescence based on three solvent extracts (methanol, ethyl acetate and water) was measured using Folin-Ciocalteu reagent method. The cytotoxic effect of the various solvent extracts and tannins on five cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB) were assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide [MTT].
Results: Ethyl acetate extract had the highest phenolic content (725.02 mg GAE/g), followed by methanol extract (627.64 mg GAE/g). Among the extracts tested, ethyl acetate extract had effective anticancer activity against A549, HepG2, HCT116 and U2OS-NKFB cells with IC
50 values of 40.1, 42.6, 40.1 and 73.4 μg/mL, respectively. Methanol extract had the strongest cytotoxic effect on SH-SY-5Y cell with IC
50 value of 31.2 μg/mL. The number of surviving cancerous cells decreased as the concentration of the ethyl acetate and methanol extracts increased.
Conclusion: The study confirms the antitumor activities of ethyl acetate and methanol extracts of
P. strobilacea infructescence. Thus, the plant extracts constitute potential therapeutic materials that require further studies and development.
