Purpose:
To investigate the therapeutic efficacy of
V. diospyroides
Symington type LS root extract as a
chemopreventive agent against various cancer cell lines.
Methods:
Acetone root extract was
evaluated for in vitro cytotoxicity against KB (oral cavity cancer),
MCF
-
7 (breast cancer), and NCI
-
H187 (small cell lung cancer), using Resazurin microplate assay
(REMA). Toxicity against a representative normal cells, Vero (African green monkey kidney),
was
assessed using green fluorescence protein (GFP)
-
based assay.
Results:
V. diospyroides root extract showed significant cytotoxic effects on KB and MCF
-
7 cell lines in
a dose
-
dependent manner with IC
50
of 35.05 ± 1.45 and 36.63 ± 3.40 μg/mL, respectivel
y. NCI
-
H187
was not
significantly inhibited (
≤ 19.39 % inhibition) at the concentrations tested. IC
50
against Vero cells
was outside the concentration range of 0.2
-
50 μg/mL.
Conclusion:
These results indicate that the root extract of
V. diospyroides has in vitro cytotoxic
effect
on human oral cavity cancer and breast cancer cells. No toxic effect on normal cells was observed.
Thus, the extract may provide bioactive substances for human cancer therapy.
