Effect of Ankaferd Blood Stopper on Early Bone Tissue Healing in Extraction Sockets: An Experimental In vivo Study

Purpose: To investigate the effect of Ankaferd blood stopper (ABS) on early bone healing of extraction sockets in rats.
Method: Twenty-eight male Wistar rats were divided into four groups. The maxillary right first molar tooth of the rats was extracted under general anesthesia. Two groups (C−1, n =6 and C−2, n =6) received saline solutions in the extraction sockets immediately and one day after the extraction, respectively, while two groups (A−1, n = 8 and A−2, n = 8) received ABS. The rats in A−1 and C−1 groups were sacrificed after 7 days of post-extraction while the rats in A−2 and C−2 groups were sacrificed after 28 days. Bone samples were taken from the maxillas, and tissues were prepared for histopathological analysis. Osteoid tissue (OT), mineralized bone tissue (MT), remaining area (RA), and inflammatory cell infiltration (ICI) were determined. The histomorphometric results were analyzed statistically using analysis of variance (ANOVA).
Results: Osteoid formation was highest in C−1 group (39.71 ± 9.68, p < 0.05). Differences in OT among other groups were not significant (p > 0.05). MT was higher in C−2 group (47.73 ± 12.15) than in other groups (p < 0.05). RA was highest in C−1 group (59.95 ± 12.75). ICI was significantly lower in control (C1 and C−2) groups than in ABS (A−1 and A−2) groups (p < 0.05).
Conclusion: ABS administered topically to extraction sockets immediately after extraction has no effect on bone healing; in fact, ABS increases inflammation in vivo.

Keywords
Ankaferd blood stopper; Wound healing; Mineralized bone tissue; Inflammatory cell infiltration; Osteoid tissue; Tooth extraction