Purpose: To investigate the expression of Myt272-3 recombinant protein and also to predict a possible
protein vaccine candidate against
Mycobacterium tuberculosis
.
Methods: Myt272-3 protein was expressed in pET30a+-Myt272-3 clone. The purity of the protein was
determined using Dynabeads® His-Tag Isolation & Pulldown. Protein sequence was analysed in silico
using bioinformatics software for the prediction of allergenicity, antigenicity, MHC-I and MHC-II binding,
and B-cell epitope binding.
Results: The candidate protein was a non-allergen with 15.19 % positive predictive value. It was also
predicted to be antigenic, with binding affinity to MHC-I and MHC-II, as well as B-cell epitope binding.
Conclusion: The predicted results obtained in this study provide a guide for practical design of a new
tuberculosis vaccine.
