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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 3, 2017, pp. 494-500
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Bioline Code: pr17064
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 3, 2017, pp. 494-500
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Preparation of Tradescantia pallida -mediated zinc oxide nanoparticles and their activity against cervical cancer cell lines
Li, Cai-yun; Zhang, Zheng-cheng; Mao, Jing-yue; Shi, Li-feng; Zheng, Ying & Quan, Jia-li
Abstract
Purpose: To synthesize zinc oxide nanoparticles (ZnO NPs) using Tradescantia pallida.
(Commelinaceae) and determine their fluorescent and cytotoxic properties.
Methods: ZnO NPs were synthesized according to a simple protocol using T. pallida aqueous leaf
extract (TPALE). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM)
were used to analyze the morphology of the ZnO NPs. X-ray diffraction (XRD) and Fourier transforminfrared
spectroscopy (FTIR) measurements were performed to determine their crystalline nature and
functional groups, respectively. Fluorescence spectroscopy was used to assess the photoluminescence
properties of ZnO NPs. Upon confirmation of ZnO NP synthesis, cytotoxicity tests were carried out
against HeLa cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Results: The agglomerated ZnO NPs were rod-shaped and had a mean particle size of 25 ± 2 nm.
Further, they exhibited good photoluminescence with correlation to ZnO crystals. MTT assay results
indicated significant cytotoxicity against HeLa cervical cancer cell line.
Conclusion: A simple approach for ZnO NP synthesis based on TPALE has been developed
successfully. The synthesized ZnO NPs demonstrate good luminescence properties and cytotoxicity
against cervical cancer line.
Keywords
Commelinaceae; Cytotoxicity; Photoluminescence; Setcreasea pallida; Setcreasea purpurea; Tradescantia pallida; ZnO nanoparticles
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