Purpose: To evaluate the activity of extracts of Sanghuangporus sp.1 fungus against pathogenic
bacteria and a breast cancer cell line.
Methods: The wild fruiting body and mycelium of Sanghuangporus sp.1 were extracted with water and
ethanol by ultrasonication extraction. The activity of the extracts against pathogenic bacteria was
determined by agar-well diffusion method while minimum inhibitory concentration (MIC) and minimum
bactericidal concentration (MBC) were obtained by agar-well diffusion and broth macrodilution methods.
The cytotoxicity of the extract against human breast cancer cell line MCF-7 was determined by
sulforhodamine B assay.
Results: Only the ethanol mycelial extract exhibited antibacterial activity. Activity was detected against
6 of the 17 test strains of bacterial pathogen. The MICs and MBCs against these 6 strains were quite
low, especially for
B. cereus
ATCC 11778 (2.5 and 2.5 mg/mL) and S. aureus (MSSA, DMST 2933, 2.5
and 5.0 mg/mL). The ethanol mycelial extract was a more potently cytotoxic against MCF-7 cells than
either the aqueous mycelial extract or the ethanol wild fruiting body extract, inhibiting cell growth at a
concentration of 250 μg/mL. The aqueous wild fruiting body extract was inactive against MCF-7 cells
when compared with untreated control groups.
Conclusion: The ethanol mycelial extract of the medicinal mushroom Sanghuangporus sp.1, obtained
by ultrasonication extraction method, exhibited potent antibacterial and anticancer activity and seems to
be a substitute for wild Sanghuangporus sp.
